Role of calcium, glutamate neurotransmission, and nitric oxide in spreading acidification and depression in the cerebellar cortex

Gang Chen, Robert L Dunbar, Wangcai Gao, Timothy J Ebner

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

This study investigated the mechanisms underlying the recently reported fast spreading acidification and transient depression in the cerebellar cortex in vivo. Spreading acidification was evoked by surface stimulation in the rat and mouse cerebellar cortex stained with the pH-sensitive dye neutral red and monitored using epifluorescent imaging. The probability of evoking spreading acidification was dependent on stimulation parameters; greater frequency and/or greater amplitude were more effective. Although activation of the parallel fibers defined the geometry of the spread, their activation alone was not sufficient, because blocking synaptic transmission with low Ca2+ prevented spreading acidification. Increased postsynaptic excitability was also a major factor. Application of either AMPA or metabotropic glutamate receptor antagonists reduced the likelihood of evoking spreading acidification, but stronger stimulation intensities were still effective. Conversely, superfusion with GABA receptor antagonists decreased the threshold for evoking spreading acidification. Blocking nitric oxide synthase (NOS) increased the threshold for spreading acidification, and nitric oxide donors lowered the threshold. However, spreading acidification could be evoked in neuronal NOS-deficient mice (B6;129S-Nos1tm1plh). The depression in cortical excitability that accompanies spreading acidification occurred in the presence of AMPA and metabotropic glutamate receptor antagonists and NOS inhibitors. These findings suggest that spreading acidification is dependent on extracellular Ca2+ and glutamate neurotransmission with a contribution from both AMPA and metabotropic glutamate receptors and is modulated by nitric oxide. Therefore, spreading acidification involves both presynaptic and postsynaptic mechanisms. We hypothesize that a regenerative process, i.e., a nonpassive process, is operative that uses the cortical architecture to account for the high speed of propagation.

Original languageEnglish (US)
Pages (from-to)9877-9887
Number of pages11
JournalJournal of Neuroscience
Volume21
Issue number24
StatePublished - Dec 15 2001

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Cerebellar Cortex
alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid
Metabotropic Glutamate Receptors
Synaptic Transmission
Glutamic Acid
Excitatory Amino Acid Antagonists
Nitric Oxide
Calcium
Nitric Oxide Synthase
GABA Antagonists
Neutral Red
Nitric Oxide Synthase Type I
Nitric Oxide Donors
Coloring Agents

Keywords

  • Calcium
  • Calcium waves
  • Cerebellum
  • Glutamate
  • Neutral red
  • Nitric oxide
  • Optical imaging
  • Rat
  • Spreading depression
  • Transgenic mouse

Cite this

Role of calcium, glutamate neurotransmission, and nitric oxide in spreading acidification and depression in the cerebellar cortex. / Chen, Gang; Dunbar, Robert L; Gao, Wangcai; Ebner, Timothy J.

In: Journal of Neuroscience, Vol. 21, No. 24, 15.12.2001, p. 9877-9887.

Research output: Contribution to journalArticle

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