Role of afferent and efferent renal nerves in the development of AngII-salt hypertension in rats

Jason D. Foss, Jessica Fiege, Yoji Shimizu, John P Collister, Tim Mayerhofer, Laurel Wood, John W Osborn Jr

Research output: Contribution to journalArticle

Abstract

Hypertension is the leading modifiable risk factor for death worldwide, yet the causes remain unclear and treatment remains suboptimal. Catheter-based renal denervation (RDNX) is a promising new treatment for resistant hypertension, but the mechanisms underlying its antihypertensive effect remain unclear. We recently found that RDNX attenuates deoxycorticosterone acetate-salt hypertension and that this is dependent on ablation of afferent renal nerves and is associated with decreased renal inflammation. To determine if this is common to other models of salt-sensitive hypertension, rats underwent complete RDNX (n = 8), selective ablation of afferent renal nerves (n = 8), or sham denervation (n = 8). Mean arterial pressure (MAP) and heart rate were measure by telemetry and rats were housed in metabolic cages for measurement of sodium and water balance. Rats were then subjected to angiotensin II (AngII)-salt hypertension (10 ng/kg/min, intravenous + 4% NaCl diet) for 2 weeks. At the end of the study, renal T-cell infiltration was quantified by flow cytometry. AngII resulted in an increase in MAP of ~50 mmHg in all three groups with no between group differences, and a transient bradycardia that was blunted by selective ablation of afferent renal nerves. Sodium and water balance were unaffected by AngII-salt treatment and similar between groups. Lastly, AngII infusion was not associated with T-cell infiltration into the kidneys, and T-cell counts were unaffected by the denervation procedures. These results suggest that AngII-salt hypertension in the rat is not associated with renal inflammation and that neither afferent nor efferent renal nerves contribute to this model.

Original languageEnglish (US)
Article numbere13602
JournalPhysiological Reports
Volume6
Issue number3
DOIs
StatePublished - Feb 1 2018

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Angiotensin II
Salts
Hypertension
Kidney
Denervation
T-Lymphocytes
Arterial Pressure
Sodium
Inflammation
Desoxycorticosterone
Telemetry
Water
Bradycardia
Antihypertensive Agents
Flow Cytometry
Acetates
Catheters
Cell Count
Heart Rate
Diet

Keywords

  • T cells
  • afferent renal nerves
  • inflammation
  • renal denervation

Cite this

Role of afferent and efferent renal nerves in the development of AngII-salt hypertension in rats. / Foss, Jason D.; Fiege, Jessica; Shimizu, Yoji; Collister, John P; Mayerhofer, Tim; Wood, Laurel; Osborn Jr, John W.

In: Physiological Reports, Vol. 6, No. 3, e13602, 01.02.2018.

Research output: Contribution to journalArticle

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