Microinjection of genetic components and dye into organotypic slices provides excellent single cell resolution for unraveling biological complexities, but is extremely difficult and time consuming to perform manually resulting in low yield and low use in the developmental biology field. We developed a computer vision guided platform to inject specimen with mRNA, and/or dye and investigated the efficiency of the process using organotypic slices of the mouse developing neocortex. We demonstrate that the system significantly increases yield of injection relative to manual use by an order of magnitude, allows for cell tracking over 0, 24, and 48 hours post injection in culture, and enables mRNA translation of injected product. The autoinjector platform thus can open the door to new types of experiments including investigating effects of mRNA concentration, and composition on cell fate, and tracking these effects on cell reprogramming and lineage.
|Original language||English (US)|
|State||Published - 2018|
|Event||2018 Design of Medical Devices Conference, DMD 2018 - Minneapolis, United States|
Duration: Apr 9 2018 → Apr 12 2018
|Other||2018 Design of Medical Devices Conference, DMD 2018|
|Period||4/9/18 → 4/12/18|
Bibliographical noteFunding Information:
We thank MnDRIVE (Minnesota’s Discovery, Research, and InnoVation Economy), Department of Mechanical Engineering, University of Minnesota, The Lions Research Building/Mcguire Translational Research Facility, and the University Imaging Center. SBK acknowledges ME department funds, MnDRIVE RSAM, McGovern Institute Neurotechnology (MINT) fund, NIH 1R21NS103098-01 and UMN medical innovation grant. G.S. was supported by the Integrative Graduate Education and ResearchTraineeship (IGERT) Fellowship in neuroengineering and the IGERT travel award.