RNA association and nucleocytoplasmic shuttling by ataxin-1

Stuart Irwin, Mark Vandelft, Deborah Pinchev, Jenny L. Howell, Joanna Graczyk, Harry T. Orr, Ray Truant

Research output: Contribution to journalArticlepeer-review

101 Scopus citations


Spinocerebellar ataxia type 1 (SCA1) is a dominant neurodegenerative disease caused by the expression of mutant ataxin-1 containing an expanded polyglutamine tract. Ataxin-1 is a nuclear protein that localizes to punctate inclusions similar to neuronal nuclear inclusions seen in many polyglutamine expansion disease proteins. We demonstrate that ataxin-1 localization to inclusions and inclusion dynamics within the nucleus are RNA and transcription dependent, but not dependent on the polyglutamine tract. Ataxin-1 nuclear inclusions are distinct from other described nuclear bodies but recruit the mRNA export factor, TAP/NXF1, in a manner that is enhanced by cell heat shock. By FRAP protein dynamic studies in live cells, we found that wild-type, but not mutant, ataxin-1 was capable of nuclear export. These results suggest that the normal role of ataxin-1 may be in RNA processing, perhaps nuclear RNA export. Thus, nuclear retention of mutant ataxin-1 may be an important toxic gain of function in SCA1 disease.

Original languageEnglish (US)
Pages (from-to)233-242
Number of pages10
JournalJournal of cell science
Issue number1
StatePublished - Jan 1 2005


  • Ataxin-1
  • Nuclear transport
  • RNA processing
  • Spinocerebellar ataxia type 1


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