Risks of Insertional Mutagenesis by DNA Transposons in Cancer Gene Therapy

Perry B. Hackett, Timothy K. Starr, Laurence J.N. Cooper

Research output: Chapter in Book/Report/Conference proceedingChapter

4 Scopus citations

Abstract

The Sleeping Beauty (SB) transposon/transposase system has recently been applied in clinical trials to redirect T-cell specificity through the addition of a transgenic cassette that drives expression of a chimeric antigen receptor. This chapter focuses on issues relating to insertional mutagenesis in the context of the plasticity of human genomes; the unexpected variability in human genomes elucidated by recent high-throughput, whole-genome sequencing; and the recently discovered high rates of remobilization of endogenous transposable elements. These findings are discussed in the context of using SB transposons to treat human disease and suggest that integration by SB transposons is not likely to induce adverse events in the clinic.

Original languageEnglish (US)
Title of host publicationTranslating Gene Therapy to the Clinic
Subtitle of host publicationTechniques and Approaches
PublisherElsevier Inc.
Pages65-83
Number of pages19
ISBN (Electronic)9780128005644
ISBN (Print)9780128005637
DOIs
StatePublished - Jan 1 2015

Keywords

  • AAPC, Artificial antigen-presenting cell
  • CAR, Chimeric antigen receptor
  • HSC, Hematopoietic stem cell
  • ITR, Inverted terminal repeat
  • LINE/SINE, Long/short interspersed elements
  • SB, Sleeping Beauty

Fingerprint Dive into the research topics of 'Risks of Insertional Mutagenesis by DNA Transposons in Cancer Gene Therapy'. Together they form a unique fingerprint.

Cite this