Rigorous swim training impairs mitochondrial function in post-ischaemic rat heart

S. B. Leichtweis, C. Leeuwenburgh, D. J. Parmelee, R. Fiebig, L. L. Ji

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

The effect of rigorous swim training (6 h day-1, 5 days week-1 for an average of 191 h) on mitochondrial respiratory function was investigated in rat heart subjected to in vivo ischaemia reperfusion (I-R). Mitochondria was isolated from the risk region of the left ventricle subjected to 60 min occlusion of the main left coronary artery followed by 30 min reperfusion. Heart weight and heart-to-body weight ratio was increased by 21 and 28% (P < 0.01), respectively, in the trained (T, n = 15) vs. control rats (C, n = 20). I-R per se showed minimal effect on heart mitochondria regardless of training status. In sham, state 4 respiration rate was 26 and 32% (P < 0.05) lower in T vs. C rats, using malate-pyruvate (M-P) and 2-oxoglutarate (OG) as substrates, respectively. Training also reduced state 3 respiration by 28% (M-P) and 50% (OG) (P < 0.01). The respiratory control index (RCI) was unaltered in T with M-P, but decreased with OG (P < 0.01). In vitro exposure to superoxide radicals severely reduced state 4 and 3 respiration and RCI, but T hearts showed greater reductions of state 4 and 3 rates than C. Mitochondria from T hearts also revealed a greater state 4 inhibition by H2O2 and HO. compared with C. A lower glutathione content and a higher γ-glutamyltranspeptidase activity (P < 0.05) was observed in T vs. C. It is concluded that rigorous swim training impairs heart mitochondrial function, making them more susceptible to in vivo and in vitro oxidative stress, and that this damaging effect may be related to a diminished glutathione reserve.

Original languageEnglish (US)
Pages (from-to)139-148
Number of pages10
JournalActa Physiologica Scandinavica
Volume160
Issue number2
DOIs
StatePublished - Jan 1 1997

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Pyruvic Acid
Reperfusion
Ketoglutaric Acids
Heart Mitochondria
Glutathione
Respiration
Ischemia
Respiratory Rate
Superoxides
Heart Ventricles
Coronary Vessels
Mitochondria
Oxidative Stress
Body Weight
Weights and Measures
malic acid
In Vitro Techniques

Keywords

  • Glutathione
  • Heart
  • Ischemia-reperfusion
  • Mitochondrial respiration
  • Oxidative damage
  • Training

Cite this

Rigorous swim training impairs mitochondrial function in post-ischaemic rat heart. / Leichtweis, S. B.; Leeuwenburgh, C.; Parmelee, D. J.; Fiebig, R.; Ji, L. L.

In: Acta Physiologica Scandinavica, Vol. 160, No. 2, 01.01.1997, p. 139-148.

Research output: Contribution to journalArticle

Leichtweis, S. B. ; Leeuwenburgh, C. ; Parmelee, D. J. ; Fiebig, R. ; Ji, L. L. / Rigorous swim training impairs mitochondrial function in post-ischaemic rat heart. In: Acta Physiologica Scandinavica. 1997 ; Vol. 160, No. 2. pp. 139-148.
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