Rickettsia peacockii, an endosymbiont of Dermacentor andersoni, does not elicit or inhibit humoral immune responses from immunocompetent D. andersoni or Ixodes scapularis cell lines

Research output: Contribution to journalArticle

11 Scopus citations

Abstract

Ixodes scapularis and Dermacentor andersoni cell lines were stimulated with heat-killed Escherichia coli and Micrococcus luteus to investigate whether infection by Rickettsia peacockii, an endosymbiont of D. andersoni, modifies humoral immune responses. Radial diffusion assays, western blotting, flow cytometry, and quantitative reverse-transcription PCR were used to determine if expression of bacteriolytic peptides, including lysozyme and defensin, was upregulated by bacterial stimulation or infection with R. peacockii. The I. scapularis line IDE12 upregulated expression of lysozyme and defensin following stimulation. The D. andersoni cell line DAE15 also expressed defensin and lysozyme, but only lysozyme was upregulated by bacterial stimulation. R. peacockii infection alone, or in cells stimulated with bacteria, did not modify defensin or lysozyme expression in either cell line. These results suggest tick endosymbionts may avoid recognition by the tick immune system, and infection may not affect humoral immune responses to bacteria not normally associated with ticks.

Original languageEnglish (US)
Pages (from-to)1095-1106
Number of pages12
JournalDevelopmental and Comparative Immunology
Volume31
Issue number11
DOIs
StatePublished - Oct 22 2007

Keywords

  • Defensin
  • Endosymbiont
  • Immune evasion
  • Immune response
  • Lysozyme
  • Real-time PCR
  • Rickettsia
  • Tick cell culture

Fingerprint Dive into the research topics of 'Rickettsia peacockii, an endosymbiont of Dermacentor andersoni, does not elicit or inhibit humoral immune responses from immunocompetent D. andersoni or Ixodes scapularis cell lines'. Together they form a unique fingerprint.

  • Cite this