Rickettsia buchneri sp. Nov., a rickettsial endosymbiont of the blacklegged tick ixodes scapularis

Timothy J Kurtti, Roderick F. Felsheim, Nicole Y. Burkhardt, Jonathan Oliver, Chan C. Heu, Ulrike G Munderloh

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

We obtained a rickettsial isolate from the ovaries of the blacklegged tick, Ixodes scapularis. The isolate (ISO7T) was grown in the Ixodes ricinus embryonic cell line IRE11. We characterized the isolate by transmission electron microscopy and gene sequencing. Phylogenetic analysis of 11 housekeeping genes demonstrated that the isolate fulfils the criteria to be classified as a representative of a novel rickettsial species closely related to ‘Rickettsia monacensis’. These rickettsiae form a clade separate from other species of rickettsiae. Gene sequences indicated that several genes important in rickettsial motility, invasiveness and temperature adaptation were mutated (e.g. sca2, rickA, hsp22, pldA and htrA). We propose the name Rickettsia buchneri sp. nov. for this bacterium that infects the ovaries of the tick I. scapularis to acknowledge the pioneering contributions of Professor Paul Buchner (1886-1978) to research on bacterial symbionts. The type strain of R. buchneri sp. nov. is strain ISO-7T (=DSM 29016T=ATCC VR- 1814T

Original languageEnglish (US)
Pages (from-to)965-970
Number of pages6
JournalInternational Journal of Systematic and Evolutionary Microbiology
Volume65
Issue number3
DOIs
StatePublished - Jan 1 2015

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Rickettsia
Ixodes
Ixodes scapularis
endosymbiont
tick
endosymbionts
Ticks
gene
Ovary
Ixodes ricinus
new species
Genes
symbionts
invasiveness
transmission electron microscopy
ticks
Essential Genes
motility
genes
symbiont

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Rickettsia buchneri sp. Nov., a rickettsial endosymbiont of the blacklegged tick ixodes scapularis. / Kurtti, Timothy J; Felsheim, Roderick F.; Burkhardt, Nicole Y.; Oliver, Jonathan; Heu, Chan C.; Munderloh, Ulrike G.

In: International Journal of Systematic and Evolutionary Microbiology, Vol. 65, No. 3, 01.01.2015, p. 965-970.

Research output: Contribution to journalArticle

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