Ribosomal Tag Pyrosequencing of DNA and RNA Reveals “Rare” Taxa with High Protein Synthesis Potential in the Sediment of a Hypersaline Lake in Western Australia

Pascal Weigold, Alexander Ruecker, Tina Loesekann-Behrens, Andreas Kappler, Sebastian Behrens

    Research output: Contribution to journalArticlepeer-review

    11 Scopus citations

    Abstract

    Little is known about the potential activity of microbial communities in hypersaline sediment ecosystems. Ribosomal tag libraries of DNA and RNA extracted from the sediment of Lake Strawbridge (Western Australia) revealed bacterial and archaeal operational taxonomic units (OTUs) with high RNA/DNA ratios providing evidence for the presence of ‘rare’ but potentially “active” taxa. Among the ‘rare’ bacterial taxa Halomonas, Salinivibrio and Idiomarina showed the highest protein synthesis potential. Rare but ‘active’ archaeal OTUs were related to the KTK 4A cluster and the Marine-Benthic-Groups B and D. We present the first molecular analysis of the microbial diversity and protein synthesis potential of rare microbial taxa in a hypersaline sediment ecosystem.

    Original languageEnglish (US)
    Pages (from-to)426-440
    Number of pages15
    JournalGeomicrobiology Journal
    Volume33
    Issue number5
    DOIs
    StatePublished - May 27 2016

    Bibliographical note

    Publisher Copyright:
    © 2016 Taylor & Francis Group, LLC.

    Keywords

    • Halomonas
    • Idiomarina
    • KTK 4A cluster
    • Marine Benthic Group B
    • Marine Benthic Group D
    • Planococcus
    • Salinivibrio
    • microbial activity
    • microbial community composition
    • microbial diversity
    • rare biosphere
    • ribosomal sequence tags
    • salt lake

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