RhoC maintains vascular homeostasis by regulating VEGF-induced signaling in endothelial cells

Luke H. Hoeppner, Sutapa Sinha, Ying Wang, Resham Bhattacharya, Shamit Dutta, Xun Gong, Victoria M. Bedell, Sandip Suresh, Changzoon Chun, Ramani Ramchandran, Stephen C. Ekker, Debabrata Mukhopadhyay

Research output: Contribution to journalArticlepeer-review

36 Scopus citations


Vasculogenesis and angiogenesis are controlled by vascular endothelial growth factor A (VEGF-A). Dysregulation of these physiological processes contributes to the pathologies of heart disease, cancer and stroke. Rho GTPase proteins play an integral role in VEGF-mediated formation and maintenance of blood vessels. The regulatory functions of RhoA and RhoB in vasculogenesis and angiogenesis arewell defined, whereas the purpose of RhoC remains poorly understood. Here, we describe how RhoC promotes vascular homeostasis by modulating endothelial cell migration, proliferation and permeability. RhoC stimulates proliferation of human umbilical vein endothelial cells (HUVECs) by stabilizing nuclear β-catenin, which promotes transcription of cyclin D1 and subsequently drives cell cycle progression. RhoC negatively regulates endothelial cell migration through MAPKs and downstream MLC2 signaling, and decreases vascular permeability through downregulation of the phospholipase Cγ (PLCγ)-Ca2+-eNOS cascade in HUVECs. Using a VEGF-inducible zebrafish (Danio rerio) model, we observed significantly less vascular permeability in RhoC morpholino (MO)- injected zebrafish than control MO-injected zebrafish. Taken together, our findings suggest that RhoC is a key regulator of vascular homeostasis in endothelial cells.

Original languageEnglish (US)
Pages (from-to)3556-3568
Number of pages13
JournalJournal of cell science
Issue number19
StatePublished - 2015

Bibliographical note

Publisher Copyright:
© 2015.


  • Endothelial cell
  • Migration
  • Permeability
  • Proliferation
  • RhoC
  • VEGF


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