Rhamm-/- fibroblasts are defective in CD44-mediated ERK1,2 motogenic signaling, leading to defective skin wound repair

Cornelia Tolg, Sara R. Hamilton, Kerry Ann Nakrieko, Fatemeh Kooshesh, Paul Walton, James B. McCarthy, Mina J. Bissell, Eva A. Turley

Research output: Contribution to journalArticlepeer-review

109 Scopus citations

Abstract

Rhamm (receptor for hyaluronan-mediated motility) is an hyaluronan binding protein with limited expression in normal tissues and high expression in advanced cancers. To understand its physiological functions and identify the molecular mechanisms underlying these functions, we created mice with a genetic deletion of Rhamm. We show that Rhamm-/- fibroblasts fail to resurface scratch wounds >3 mm or invade hyaluronan-supplemented collagen gels in culture. We identify a requirement for Rhamm in the localization of CD44 to the cell surface, formation of CD44-ERK1,2 (extracellular-regulated kinase 1,2) complexes, and activation/subcellular targeting of ERK1,2 to the cell nucleus. We also show that cell surface Rhamm, restricted to the extracellular compartment by linking recombinant protein to beads, and expression of mutant active mitogen-activated kinase kinase 1 (Mek1) are sufficient to rescue aberrant signaling through CD44-ERK1,2 complexes in Rh-/- fibroblasts. ERK1,2 activation and fibroblast migration/differentiation is also defective during repair of Rh-/- excisional skin wounds and results in aberrant granulation tissue in vivo. These results identify Rhamm as an essential regulator of CD44-ERK1,2 fibroblast motogenic signaling required for wound repair.

Original languageEnglish (US)
Pages (from-to)1017-1028
Number of pages12
JournalJournal of Cell Biology
Volume175
Issue number6
DOIs
StatePublished - Dec 18 2006

Bibliographical note

Copyright:
Copyright 2008 Elsevier B.V., All rights reserved.

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