RFLP mapping of an aphid resistance gene in cowpea (Vigna unguiculata L. Walp)

G. O. Myers; C. A. Fatokun; N. D. Young

RFLP mapping of an aphid resistance gene in cowpea (Vigna unguiculata L. Walp)

G. O. Myers, C. A. Fatokun, N. D. Young

Research output: Contribution to journal › Article › peer-review

Abstract

Restriction fragment length polymorphism (RFLP) analysis has several advantages over traditional methods of genetic linkage mapping, one of these being the starting point for map-based cloning. The recent development of an RFLP map of cowpea (Vigna unguiculata L. Walp) has allowed the investigation of associations between genes of interest and RFLP markers. A cross between an aphid (Aphis craccivora Koch) resistant cultivated cowpea, IT84S-2246-4, and an aphid susceptible wild cowpea, NI 963, was screened for both aphid phenotype and RFLP marker segregation. One RFLP marker, bg4D9b, was found to be tightly linked to the aphid resistance gene (Rac₁) and several flanking markers in the same linkage group (linkage group 1) were also identified. The close association of Rac₁ and RFLP bg4D9b presents a real potential for cloning this insect resistance gene.

Original language	English (US)
Pages (from-to)	181-187
Number of pages	7
Journal	Euphytica
Volume	91
Issue number	2
State	Published - 1996
Externally published	Yes

Keywords

Aphis craccivora
DNA markers
Vigna unguiculata
cowpea
insect
legume
restriction fragment length polymorphisms

Find It

Find It at U of M Libraries

Cite this

@article{2e04e47b5e14437db4d6016a6f31d0a2,

title = "RFLP mapping of an aphid resistance gene in cowpea (Vigna unguiculata L. Walp)",

abstract = "Restriction fragment length polymorphism (RFLP) analysis has several advantages over traditional methods of genetic linkage mapping, one of these being the starting point for map-based cloning. The recent development of an RFLP map of cowpea (Vigna unguiculata L. Walp) has allowed the investigation of associations between genes of interest and RFLP markers. A cross between an aphid (Aphis craccivora Koch) resistant cultivated cowpea, IT84S-2246-4, and an aphid susceptible wild cowpea, NI 963, was screened for both aphid phenotype and RFLP marker segregation. One RFLP marker, bg4D9b, was found to be tightly linked to the aphid resistance gene (Rac1) and several flanking markers in the same linkage group (linkage group 1) were also identified. The close association of Rac1 and RFLP bg4D9b presents a real potential for cloning this insect resistance gene.",

keywords = "Aphis craccivora, DNA markers, Vigna unguiculata, cowpea, insect, legume, restriction fragment length polymorphisms",

author = "Myers, {G. O.} and Fatokun, {C. A.} and Young, {N. D.}",

year = "1996",

language = "English (US)",

volume = "91",

pages = "181--187",

journal = "Euphytica",

issn = "0014-2336",

publisher = "Springer Science and Business Media B.V.",

number = "2",

}

TY - JOUR

T1 - RFLP mapping of an aphid resistance gene in cowpea (Vigna unguiculata L. Walp)

AU - Myers, G. O.

AU - Fatokun, C. A.

AU - Young, N. D.

PY - 1996

Y1 - 1996

N2 - Restriction fragment length polymorphism (RFLP) analysis has several advantages over traditional methods of genetic linkage mapping, one of these being the starting point for map-based cloning. The recent development of an RFLP map of cowpea (Vigna unguiculata L. Walp) has allowed the investigation of associations between genes of interest and RFLP markers. A cross between an aphid (Aphis craccivora Koch) resistant cultivated cowpea, IT84S-2246-4, and an aphid susceptible wild cowpea, NI 963, was screened for both aphid phenotype and RFLP marker segregation. One RFLP marker, bg4D9b, was found to be tightly linked to the aphid resistance gene (Rac1) and several flanking markers in the same linkage group (linkage group 1) were also identified. The close association of Rac1 and RFLP bg4D9b presents a real potential for cloning this insect resistance gene.

AB - Restriction fragment length polymorphism (RFLP) analysis has several advantages over traditional methods of genetic linkage mapping, one of these being the starting point for map-based cloning. The recent development of an RFLP map of cowpea (Vigna unguiculata L. Walp) has allowed the investigation of associations between genes of interest and RFLP markers. A cross between an aphid (Aphis craccivora Koch) resistant cultivated cowpea, IT84S-2246-4, and an aphid susceptible wild cowpea, NI 963, was screened for both aphid phenotype and RFLP marker segregation. One RFLP marker, bg4D9b, was found to be tightly linked to the aphid resistance gene (Rac1) and several flanking markers in the same linkage group (linkage group 1) were also identified. The close association of Rac1 and RFLP bg4D9b presents a real potential for cloning this insect resistance gene.

KW - Aphis craccivora

KW - DNA markers

KW - Vigna unguiculata

KW - cowpea

KW - insect

KW - legume

KW - restriction fragment length polymorphisms

UR - http://www.scopus.com/inward/record.url?scp=0029799442&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029799442&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:0029799442

SN - 0014-2336

VL - 91

SP - 181

EP - 187

JO - Euphytica

JF - Euphytica

IS - 2

ER -

RFLP mapping of an aphid resistance gene in cowpea (Vigna unguiculata L. Walp)

Abstract

Keywords

Find It

Other files and links

Fingerprint

Cite this