RFLP mapping of a major bruchid resistance gene in mungbean (Vigna radiata, L. Wilczek)

N. D. Young, L. Kumar, D. Menancio-Hautea, D. Danesh, N. S. Talekar, S. Shanmugasundarum, D. H. Kim

Research output: Contribution to journalArticlepeer-review

103 Scopus citations


Bruchids (genus Callosobruchus) are among the most destructive insect pests of mungbeans and other members of the genus, Vigna. Genetic resistance to bruchids was previously identified in a wild mungbean relative, TC1966. To analyze the underlying genetics, accelerate breeding, and provide a basis for map-based cloning of this gene, we have mapped the TC1966 bruchid resistance gene using restriction fragment length polymorphism (RFLP) markers. Fifty-eight F2 progeny from a cross between TC1966 and a susceptible mungbean cultivar were analyzed with 153 RFLP markers. Resistance mapped to a single locus on linkage group VIII, approximately 3.6 centimorgans from the nearest RFLP marker. Because the genome of mungbean is relatively small (estimated to be between 470 and 560 million base pairs), this RFLP marker may be suitable as a starting point for chromosome walking. Based on RFLP analysis, an individual was also identified in the F2 population that retained the bruchid resistance gene within a tightly linked double crossover. This individual will be valuable in developing resistant mungbean lines free of linkage drag.

Original languageEnglish (US)
Pages (from-to)839-844
Number of pages6
JournalTheoretical and Applied Genetics
Issue number7-8
StatePublished - Sep 1992


  • Callosobruchus
  • DNA Markers
  • Insect
  • Legume
  • Restriction fragment length polymorphisms


Dive into the research topics of 'RFLP mapping of a major bruchid resistance gene in mungbean (Vigna radiata, L. Wilczek)'. Together they form a unique fingerprint.

Cite this