Abstract
The partitioning and absorption mechanisms of retention in reversed-phase liquid chromtography (RPLC) were examined based on the free energy of transfer of a methylene group from organic aqueous mixtures to bulk hexadecane. Retention on monomeric bonded phases with octyl chains or longer are dominated by a partitioning-like mechanism. An adsorption-like mechanism contributes to retention in monomeric bonded phases with short bonded chains or with low surface coverage density. The retention mechanism becomes more adsorption-like as the bonded chains decrease.
Original language | English (US) |
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Pages (from-to) | 1-12 |
Number of pages | 12 |
Journal | Journal of Chromatography A |
Volume | 775 |
Issue number | 1-2 |
DOIs | |
State | Published - Jul 18 1997 |
Bibliographical note
Funding Information:This work was supported by a grant from the National Science Foundation.
Keywords
- Adsorption
- Partitioning
- Retention mechanisms
- Solvophobic theory
- Stationary phases, LC