Revised guides for organ sampling and trimming in rats and mice - Part 2: A joint publication of the RITA*) and NACAD**) groups

Birgit Kittel, Christine Ruehl-Fehlert, Gerd Morawietz, Jan Klapwijk, Michael R. Elwell, Barbara Lenz, M. Gerard O'Sullivan, Daniel R. Roth, Peter F. Wadsworth

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194 Scopus citations


This is the second part of a series of three articles on trimming instructions of rat and mouse protocol organs and tissues in regulatory type toxicity studies, covering the respiratory, male and female genital, and the endocrine systems. The article is based on the experience of the European RITA and American NACAD working groups and is an extended revision of trimming guides published in 1995 (BAHNEMANN et al.). The optimum localization for tissue preparation, the sample size, the direction of sectioning and the number of sections to be prepared is described organ by organ. These descriptions are illustrated for each organ by a schematic drawing and/or a macro-photograph showing the plane of section as well as a low magnification of the H&E stained slide demonstrating the optimum "end-product". The objectives of this work, as addressed in detail in the first part (RUEHL-FEHLERT et al. 2003), are to standardize tissue sampling and trimming, to improve the comparability of historical data obtained from different studies and different laboratories, ensure the presence of all relevant target sites for histopathological evaluation and provide technical advice for preparatory techniques during necropsy, fixation and trimming.

Original languageEnglish (US)
Pages (from-to)413-431
Number of pages19
JournalExperimental and Toxicologic Pathology
Issue number6
StatePublished - Jul 2004
Externally publishedYes


  • Bronchiole
  • Bronchus
  • Coagulating gland
  • Epididymis
  • Guidelines
  • Larynx
  • Lung
  • Mouse
  • Nasal cavity
  • Nasopharynx
  • Ovary
  • Oviduct
  • Paranasal sinus
  • Prostate
  • RITA
  • Rat
  • Rete testis
  • Seminal vesicle
  • Standardization
  • Testis
  • Trachea
  • Trimming
  • Uterine cervix
  • Uterus


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