The analysis of isolated organelles is one of the pillars of modern bioanalytical chemistry. This review describes recent developments on the isolation and characterization of isolated organelles both from living organisms and cell cultures. Salient reports on methods to release organelles focused on reproducibility and yield, membrane isolation, and integrated devices for organelle release. New developments on organelle fractionation after their isolation were on the topics of centrifugation, immunocapture, free flow electrophoresis, flow field-flow fractionation, fluorescence activated organelle sorting, laser capture microdissection, and dielectrophoresis. New concepts on characterization of isolated organelles included atomic force microscopy, optical tweezers combined with Raman spectroscopy, organelle sensors, flow cytometry, capillary electrophoresis, and microfluidic devices.
Bibliographical noteFunding Information:
The authors acknowledge support from NIH , grant no. R01AG020866 (E.A.), The Center for Analysis of Biomolecular Signaling at the University of Minnesota-Twin Cities (C.S.), P206/12/G014 Center for Advanced Bioanalytical Technologies, Czech Science Foundation (V.K.), and the Republic Institutional research plan No. AV0Z40310501, Academy of Sciences of the Czech Republic (V.K.).
Chad P. Satori is a Ph.D. candidate in the Department of Chemistry at the University of Minnesota, Twin-Cities. He is a recipient of a 2011 Baxter Young Investigator Award, has been a trainee of the University of Minnesota Biotechnology Training grant, and is a trainee of the Center for Analysis of Biomolecular Signaling. His research focuses on the development of methods to acquire highly enriched organelle fractions and the development of methods to analyze and characterize organelles and their properties.
- Atomic force microscopy
- Organelle isolation
- Organelle purification