Biomedical applications of light scattering have traditionally relied on Mie theory as a theoretical basis for analyzing experimental measurements. While this approach is computationally accessible, the use of Mie theory cannot always account for the spheroidal nature of biological scatterers such as cell nuclei. As an alternative, we have developed light scattering codes based on the T-matrix formalism to analyze angle-resolved light scattering measurements. In this paper, we discuss the development of computer codes to create a database of scattering profiles, validation of the database using experimental measurements of tissue phantoms containing spheroidal scatterers, and recent applications of the approach to analyze the structure of cell nuclei. The reviewed studies show that while the T-matrix approach adds important information for some light scattering studies, the additional computational cost may not always be justified when compared to the Mie theory.
|Original language||English (US)|
|Number of pages||9|
|Journal||IEEE Journal on Selected Topics in Quantum Electronics|
|State||Published - Jul 2010|
Bibliographical noteFunding Information:
Manuscript received August 2, 2009; revised August 26, 2009; accepted August 27, 2009. Date of publication October 6, 2009; date of current version August 6, 2010. This work was supported by the National Institutes of Health under Grant NCI R33-CA109907 and Grant NCI R21CA120128, by the National Science Foundation under Grant BES 03-48204, and by the Department of Defense under Grant BC073290.
- Biomedical imaging
- Mie theory
- light scattering