Review of the application of T-matrix calculations for determining the structure of cell nuclei with angle-resolved light scattering measurements

Michael G. Giacomelli, Kevin J. Chalut, Julie H. Ostrander, Adam Wax

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Biomedical applications of light scattering have traditionally relied on Mie theory as a theoretical basis for analyzing experimental measurements. While this approach is computationally accessible, the use of Mie theory cannot always account for the spheroidal nature of biological scatterers such as cell nuclei. As an alternative, we have developed light scattering codes based on the T-matrix formalism to analyze angle-resolved light scattering measurements. In this paper, we discuss the development of computer codes to create a database of scattering profiles, validation of the database using experimental measurements of tissue phantoms containing spheroidal scatterers, and recent applications of the approach to analyze the structure of cell nuclei. The reviewed studies show that while the T-matrix approach adds important information for some light scattering studies, the additional computational cost may not always be justified when compared to the Mie theory.

Original languageEnglish (US)
Article number5280380
Pages (from-to)900-908
Number of pages9
JournalIEEE Journal on Selected Topics in Quantum Electronics
Volume16
Issue number4
DOIs
StatePublished - Jul 1 2010

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Light scattering
Mie scattering
light scattering
Cells
nuclei
scattering
matrices
Scattering
Tissue
formalism
costs
computer programs
profiles
Costs

Keywords

  • Biomedical imaging
  • Mie theory
  • T-matrix
  • light scattering

Cite this

Review of the application of T-matrix calculations for determining the structure of cell nuclei with angle-resolved light scattering measurements. / Giacomelli, Michael G.; Chalut, Kevin J.; Ostrander, Julie H.; Wax, Adam.

In: IEEE Journal on Selected Topics in Quantum Electronics, Vol. 16, No. 4, 5280380, 01.07.2010, p. 900-908.

Research output: Contribution to journalArticle

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