Reversible Re-programing of Cell-Cell Interactions

Kari Gabrielse, Amit Gangar, Nigam Kumar, Jae Chul Lee, Adrian Fegan, Jing Jing Shen, Qing Li, Daniel Vallera, Carston R. Wagner

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

The ability to engineer and re-program the surfaces of cells would provide an enabling synthetic biological method for the design of cell- and tissue-based therapies. A new cell surface-engineering strategy is described that uses lipid-chemically self-assembled nanorings (lipid-CSANs) that can be used for the stable and reversible modification of any cell surface with a molecular reporter or targeting ligand. In the presence of a non-toxic FDA-approved drug, the nanorings were quickly disassembled and the cell-cell interactions reversed. Similar to T-cells genetically engineered to express chimeric antigen receptors (CARS), when activated peripheral blood mononuclear cells (PBMCs) were functionalized with the anti-EpCAM-lipid-CSANs, they were shown to selectively kill antigen-positive cancer cells. Taken together, these results demonstrate that lipid-CSANs have the potential to be a rapid, stable, and general method for the reversible engineering of cell surfaces and cell-cell interactions. Lipid chemically self-assembled nanorings (lipid-CSANs) can be used for the stable and reversible modification of any cell surface with a molecular reporter or targeting ligand. In the presence of the antibacterial drug trimethoprim the nanorings were quickly disassembled and the cell-cell interactions reversed.

Original languageEnglish (US)
Pages (from-to)5112-5116
Number of pages5
JournalAngewandte Chemie - International Edition
Volume53
Issue number20
DOIs
StatePublished - May 12 2014

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Nanorings
Lipids
Antigens
Ligands
Antigen Receptors
Trimethoprim
T-cells
Pharmaceutical Preparations
Blood
Cells
Tissue
Engineers

Keywords

  • cell-cell interactions
  • chimeric antigen receptors
  • nanoparticles
  • oligomerization
  • self-assembly

Cite this

Gabrielse, K., Gangar, A., Kumar, N., Lee, J. C., Fegan, A., Shen, J. J., ... Wagner, C. R. (2014). Reversible Re-programing of Cell-Cell Interactions. Angewandte Chemie - International Edition, 53(20), 5112-5116. https://doi.org/10.1002/anie.201310645

Reversible Re-programing of Cell-Cell Interactions. / Gabrielse, Kari; Gangar, Amit; Kumar, Nigam; Lee, Jae Chul; Fegan, Adrian; Shen, Jing Jing; Li, Qing; Vallera, Daniel; Wagner, Carston R.

In: Angewandte Chemie - International Edition, Vol. 53, No. 20, 12.05.2014, p. 5112-5116.

Research output: Contribution to journalArticle

Gabrielse, K, Gangar, A, Kumar, N, Lee, JC, Fegan, A, Shen, JJ, Li, Q, Vallera, D & Wagner, CR 2014, 'Reversible Re-programing of Cell-Cell Interactions', Angewandte Chemie - International Edition, vol. 53, no. 20, pp. 5112-5116. https://doi.org/10.1002/anie.201310645
Gabrielse K, Gangar A, Kumar N, Lee JC, Fegan A, Shen JJ et al. Reversible Re-programing of Cell-Cell Interactions. Angewandte Chemie - International Edition. 2014 May 12;53(20):5112-5116. https://doi.org/10.1002/anie.201310645
Gabrielse, Kari ; Gangar, Amit ; Kumar, Nigam ; Lee, Jae Chul ; Fegan, Adrian ; Shen, Jing Jing ; Li, Qing ; Vallera, Daniel ; Wagner, Carston R. / Reversible Re-programing of Cell-Cell Interactions. In: Angewandte Chemie - International Edition. 2014 ; Vol. 53, No. 20. pp. 5112-5116.
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