Isocratic systems of varying suitability are reported for reversed- phase HPLC of lantadenes, the pentacyclic triterpenoids from the hepatotoxic plant Lantana camara var. aculeata. The column used was a Nova-Pak C18 (250x4.6 mm). The detection was at 210 nm and 240 nm. The mobile phase acetonitrile-water-acetic acid (80:20:0.01) provided baseline separation of all the lantadenes except lantadenes A and C. The mobile phase methanol- water-acetic acid (85:15:0.01) also provided resolution of all the lantadenes but the retention time windows were very broad. The mobile phase methanol- acetonitrile-water-acetic acid (68:20:12:0.01) has been found to be the most suitable for the separation and quantitation of lantadenes.
Bibliographical noteFunding Information:
This work has been supported by a research grant from the Department of Science and Technology, New Delhi, India. S.S. thanks the DST for the award of a junior research fellowship. The authors are grateful to Dr. Kiran Singh, Director of the Institute for the facilities and to Dr. B. Singh, Scientist-in-charge for helpful discussions.
- Lantana camara