Reverse zymography studies of protease inhibitors in the secretions of different lobes of rat prostate

Michael J. Wilson, C. Casey, M. Woodson, A. A. Sinha

Research output: Contribution to journalArticle

Abstract

The purpose of this study was to determine whether protease inhibitors were a constituent of secretions from the different lobes of the rat prostate. A reverse zymography method was used employing gelatin substrate- SDS-polyacrylamide gel electrophoresis to detect inhibitors of trypsin in secretions of the ventral, lateral, and dorsal prostate lobes of the Sprague- Dawley rat. Inhibitors of approximately 34 and 63 kDa were detected in ventral prostate secretion and of about 63 and 73 kDa were found in lateral lobe secretion. However, no inhibitor activities were detected in secretions of the dorsal lobe. The protease inhibitors of ventral prostate secretion were partially purified by preparatory isoelectric focusing and SDS- polyacrylamide gel electrophoresis. The 34-kDa (pI 5.6-6.4) inhibitor had a higher activity against trypsin, whereas the 63-kDa (pI 6.4-7.0) inhibitor was more active against chymotrypsin. The rat prostate appears to have a lobe-specific distribution of secretory serine protease inhibitors.

Original languageEnglish (US)
Pages (from-to)109-118
Number of pages10
JournalArchives of Andrology
Volume42
Issue number2
StatePublished - 1999

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Protease Inhibitors
Prostate
Polyacrylamide Gel Electrophoresis
Serine Proteinase Inhibitors
Trypsin Inhibitors
Chymotrypsin
Isoelectric Focusing
Gelatin
Trypsin
Sprague Dawley Rats

Keywords

  • Dorsal prostate
  • Lateral prostate
  • Secretion
  • Serine protease inhibitor
  • Ventral prostate

Cite this

Wilson, M. J., Casey, C., Woodson, M., & Sinha, A. A. (1999). Reverse zymography studies of protease inhibitors in the secretions of different lobes of rat prostate. Archives of Andrology, 42(2), 109-118.

Reverse zymography studies of protease inhibitors in the secretions of different lobes of rat prostate. / Wilson, Michael J.; Casey, C.; Woodson, M.; Sinha, A. A.

In: Archives of Andrology, Vol. 42, No. 2, 1999, p. 109-118.

Research output: Contribution to journalArticle

Wilson, MJ, Casey, C, Woodson, M & Sinha, AA 1999, 'Reverse zymography studies of protease inhibitors in the secretions of different lobes of rat prostate', Archives of Andrology, vol. 42, no. 2, pp. 109-118.
Wilson, Michael J. ; Casey, C. ; Woodson, M. ; Sinha, A. A. / Reverse zymography studies of protease inhibitors in the secretions of different lobes of rat prostate. In: Archives of Andrology. 1999 ; Vol. 42, No. 2. pp. 109-118.
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