Reverse transcriptase from avian myeloblastosis virus

A zinc metalloenzyme

D. S. Auld, H. Kawaguchi, D. M. Livingston, B. L. Vallee

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Previous postulates of a relationship between a zinc enzyme and the leukemic process (1,2) have led to the identification of the RNA dependent DNA polymerase -- reverse transcriptase -- of avian myeloblastosis virus as a zinc metalloenzyme. Microwave induced emission spectrometry provides a microanalytical system capable of measuring precisely 10-11 to 10-14 g atoms of metal in microgram amounts of enzyme, orders of magnitude more sensitive than other, conventional methods. The chromatographic fraction with highest enzymatic activity contains 1.5 × 10-11 g atoms of zinc per 1.4 μg of protein, corresponding to 1.7 to 1.9 g atoms of zinc per mole of enzyme for a molecular weight previously determined either as 1.6 or 1.8 × 105. The Zn/activity ratio is constant in the active fractions. Copper, iron and manganese are absent, i.e., at or below their limits of detection, 10-13 to 10-14 g atoms. Agents known to chelate zinc inhibit the enzyme while their nonchelating isomers do not. The data underline the participation of zinc in nucleic acid metabolism and bear importantly upon the lesions which accompany leukemia and zinc deficiency.

Original languageEnglish (US)
Pages (from-to)967-972
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume57
Issue number4
DOIs
StatePublished - Apr 23 1974

Fingerprint

Avian Myeloblastosis Virus
RNA-Directed DNA Polymerase
Viruses
Zinc
Atoms
Enzymes
Manganese
Microwaves
Metabolism
Isomers
Nucleic Acids
Spectrometry
Limit of Detection
Copper
Spectrum Analysis
Leukemia
Iron
Molecular Weight
Metals
Molecular weight

Cite this

Reverse transcriptase from avian myeloblastosis virus : A zinc metalloenzyme. / Auld, D. S.; Kawaguchi, H.; Livingston, D. M.; Vallee, B. L.

In: Biochemical and Biophysical Research Communications, Vol. 57, No. 4, 23.04.1974, p. 967-972.

Research output: Contribution to journalArticle

Auld, D. S. ; Kawaguchi, H. ; Livingston, D. M. ; Vallee, B. L. / Reverse transcriptase from avian myeloblastosis virus : A zinc metalloenzyme. In: Biochemical and Biophysical Research Communications. 1974 ; Vol. 57, No. 4. pp. 967-972.
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