Reversal of secondary lesions of diabetes in rats by pancreatic islet transplantation

D. E.R. Sutherland, Michael W Steffes, Michael Mauer, John S Najarian

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Inbred Lewis rats were made diabetic by streptozotocin. Six to 10 mth after the induction of diabetes, each rat underwent a renal biopsy and received an intraperitoneal transplat of isologous neonatal pancreatic islet tissue. Serial blood glucose determinations were performed to confirm amelioration of the diabetes. In 7 rats, renal biopsy specimens were obtained weekly for 3 wk and then every other week for up to 9 wk following islet transplantation. Biopsies were also done on age matched normal control and untreated diabetic animals. All biopsy specimens were examined by light microscopy and by immunofluorescent microscopy with staining for IgG, IgM, and B1C. The glomerular lesions secondary to diabetes mellitus in the rat can be arrested or reversed when a return to the normal metabolic state is effected by pancreatic islet transplantation. Most likely, glomerular mesangial function is deranged when exposed to the diabetic milieu, and macromolecules, which are normally cleared in the mesangium, and accumulate in noxious quantities. Cure of the diabetic condition restores normal mesangial function. Human and rat diabetic glomerular lesions are similar. These observations provide a rational basis for attempting to halt the progression of diabetic lesions in man by pancreas or islet transplantation.

Original languageEnglish (US)
Pages (from-to)309-311
Number of pages3
JournalSurgical Forum
VolumeVol 25
StatePublished - Jan 1 1974

Fingerprint

Islets of Langerhans Transplantation
Biopsy
Microscopy
Inbred Lew Rats
Kidney
Pancreas Transplantation
Streptozocin
Islets of Langerhans
Immunoglobulin M
Blood Glucose
Diabetes Mellitus
Immunoglobulin G
Staining and Labeling
Light

Cite this

Reversal of secondary lesions of diabetes in rats by pancreatic islet transplantation. / Sutherland, D. E.R.; Steffes, Michael W; Mauer, Michael; Najarian, John S.

In: Surgical Forum, Vol. Vol 25, 01.01.1974, p. 309-311.

Research output: Contribution to journalArticle

@article{636b088720b742a4a340d1f0ac8e1f76,
title = "Reversal of secondary lesions of diabetes in rats by pancreatic islet transplantation",
abstract = "Inbred Lewis rats were made diabetic by streptozotocin. Six to 10 mth after the induction of diabetes, each rat underwent a renal biopsy and received an intraperitoneal transplat of isologous neonatal pancreatic islet tissue. Serial blood glucose determinations were performed to confirm amelioration of the diabetes. In 7 rats, renal biopsy specimens were obtained weekly for 3 wk and then every other week for up to 9 wk following islet transplantation. Biopsies were also done on age matched normal control and untreated diabetic animals. All biopsy specimens were examined by light microscopy and by immunofluorescent microscopy with staining for IgG, IgM, and B1C. The glomerular lesions secondary to diabetes mellitus in the rat can be arrested or reversed when a return to the normal metabolic state is effected by pancreatic islet transplantation. Most likely, glomerular mesangial function is deranged when exposed to the diabetic milieu, and macromolecules, which are normally cleared in the mesangium, and accumulate in noxious quantities. Cure of the diabetic condition restores normal mesangial function. Human and rat diabetic glomerular lesions are similar. These observations provide a rational basis for attempting to halt the progression of diabetic lesions in man by pancreas or islet transplantation.",
author = "Sutherland, {D. E.R.} and Steffes, {Michael W} and Michael Mauer and Najarian, {John S}",
year = "1974",
month = "1",
day = "1",
language = "English (US)",
volume = "Vol 25",
pages = "309--311",
journal = "Surgical Forum",
issn = "0071-8041",
publisher = "American College of Surgeons",

}

TY - JOUR

T1 - Reversal of secondary lesions of diabetes in rats by pancreatic islet transplantation

AU - Sutherland, D. E.R.

AU - Steffes, Michael W

AU - Mauer, Michael

AU - Najarian, John S

PY - 1974/1/1

Y1 - 1974/1/1

N2 - Inbred Lewis rats were made diabetic by streptozotocin. Six to 10 mth after the induction of diabetes, each rat underwent a renal biopsy and received an intraperitoneal transplat of isologous neonatal pancreatic islet tissue. Serial blood glucose determinations were performed to confirm amelioration of the diabetes. In 7 rats, renal biopsy specimens were obtained weekly for 3 wk and then every other week for up to 9 wk following islet transplantation. Biopsies were also done on age matched normal control and untreated diabetic animals. All biopsy specimens were examined by light microscopy and by immunofluorescent microscopy with staining for IgG, IgM, and B1C. The glomerular lesions secondary to diabetes mellitus in the rat can be arrested or reversed when a return to the normal metabolic state is effected by pancreatic islet transplantation. Most likely, glomerular mesangial function is deranged when exposed to the diabetic milieu, and macromolecules, which are normally cleared in the mesangium, and accumulate in noxious quantities. Cure of the diabetic condition restores normal mesangial function. Human and rat diabetic glomerular lesions are similar. These observations provide a rational basis for attempting to halt the progression of diabetic lesions in man by pancreas or islet transplantation.

AB - Inbred Lewis rats were made diabetic by streptozotocin. Six to 10 mth after the induction of diabetes, each rat underwent a renal biopsy and received an intraperitoneal transplat of isologous neonatal pancreatic islet tissue. Serial blood glucose determinations were performed to confirm amelioration of the diabetes. In 7 rats, renal biopsy specimens were obtained weekly for 3 wk and then every other week for up to 9 wk following islet transplantation. Biopsies were also done on age matched normal control and untreated diabetic animals. All biopsy specimens were examined by light microscopy and by immunofluorescent microscopy with staining for IgG, IgM, and B1C. The glomerular lesions secondary to diabetes mellitus in the rat can be arrested or reversed when a return to the normal metabolic state is effected by pancreatic islet transplantation. Most likely, glomerular mesangial function is deranged when exposed to the diabetic milieu, and macromolecules, which are normally cleared in the mesangium, and accumulate in noxious quantities. Cure of the diabetic condition restores normal mesangial function. Human and rat diabetic glomerular lesions are similar. These observations provide a rational basis for attempting to halt the progression of diabetic lesions in man by pancreas or islet transplantation.

UR - http://www.scopus.com/inward/record.url?scp=0016337341&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0016337341&partnerID=8YFLogxK

M3 - Article

VL - Vol 25

SP - 309

EP - 311

JO - Surgical Forum

JF - Surgical Forum

SN - 0071-8041

ER -