Retroviral vectors for analysis of viral mutagenesis and recombination

Jonathan M.O. Rawson, Louis M. Mansky

Research output: Contribution to journalReview article

2 Scopus citations

Abstract

Retrovirus population diversity within infected hosts is commonly high due in part to elevated rates of replication, mutation, and recombination. This high genetic diversity often complicates the development of effective diagnostics, vaccines, and antiviral drugs. This review highlights the diverse vectors and approaches that have been used to examine mutation and recombination in retroviruses. Retroviral vectors for these purposes can broadly be divided into two categories: those that utilize reporter genes as mutation or recombination targets and those that utilize viral genes as targets of mutation or recombination. Reporter gene vectors greatly facilitate the detection, quantification, and characterization of mutants and/or recombinants, but may not fully recapitulate the patterns of mutagenesis or recombination observed in native viral gene sequences. In contrast, the detection of mutations or recombination events directly in viral genes is more biologically relevant but also typically more challenging and inefficient. We will highlight the advantages and disadvantages of the various vectors and approaches used as well as propose ways in which they could be improved.

Original languageEnglish (US)
Pages (from-to)3612-3642
Number of pages31
JournalViruses
Volume6
Issue number9
DOIs
StatePublished - Sep 24 2014

Keywords

  • Evolution
  • Lentivirus
  • Mutation
  • Recombination
  • Retroviral vector
  • Retrovirus
  • Reverse transcription

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