Retinoic acid inhibits cell growth in HPV negative cervical carcinoma cells by induction of insulin-like growth factor binding protein-5 (IGFBP-5) secretion

Hiromi Higo, Cunming Duan, David R. Clemmons, Brian Herman

Research output: Contribution to journalArticlepeer-review

20 Scopus citations

Abstract

Retinoids have been demonstrated to inhibit epithelial cell growth and differentiation. We examined the anti-proliferative effects of retinoic acid (RA) in an HPV positive and negative cervical carcinoma cell line. Our findings indicate that HPV-negative C33A cervical carcinoma cells are more sensitive to the growth inhibitory activity of retinoic acid (RA) than are HPV-positive CaSki cervical carcinoma cells. However, conditioned medium from RA-treated C33A cells displayed strong growth inhibitory activity in both C33A and CaSki cells. Since RA has been shown to modulate the expression of insulin-like growth factor binding proteins (IGFBPs) in many cells, we examined RA regulated expression of IGFBPs in medium isolated from RA treated C33A cells. IGFBP-5 was detectable in medium from C33A cells exposed to RA, and addition of purified exogenous IGFBP-5 resulted in growth inhibition of C33A cells. These results indicate that RA exerts it's anti-neoplastic effect in HPV negative cervical carcinoma cells via the overproduction of IGFBP-5.

Original languageEnglish (US)
Pages (from-to)706-709
Number of pages4
JournalBiochemical and Biophysical Research Communications
Volume239
Issue number3
DOIs
StatePublished - Oct 29 1997
Externally publishedYes

Bibliographical note

Funding Information:
This work was supported by Grants AG07218 and AG13637 from the NIH. The authors are grateful to Ted Busby for puri®ed IGFBP-5, and to Dr. Kenichi Higo for useful comments on the manuscript.

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