Retinoic acid-induced expression of tissue transglutaminase in human promyelocytic leukemia (HL-60) cells

P. J A Davies, M. P. Murtaugh, W. T. Moore, G. S. Johnson, D. Lucas

Research output: Contribution to journalArticlepeer-review

17 Scopus citations

Abstract

Addition of retinoic acid to human promyelocytic leukemia cells results in a dramatic increase in cellular transglutaminase activity. This increase is due to the induction of a specific intracellular transglutaminase, tissue transglutaminase. Retinoic acid-induced expression of tissue transglutaminase is potentiated by analogues of cyclic AMP. The induction of the enzyme can be detected within 6 h of the addition of the retinoid to the cell and results in increases of the enzyme of at least 50-fold. The induction of HL-60 transglutaminase is a specific response of the cells to retinoic acid and is not seen with other agents that induce HL-60 differentiation. We believe that the induction of tissue transglutaminase is a useful index of the early events in retinoid-regulated gene expression in both normal and transformed cells.

Original languageEnglish (US)
Pages (from-to)5166-5174
Number of pages9
JournalJournal of Biological Chemistry
Volume260
Issue number8
StatePublished - 1985

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