Retention mechanism in reversed-phase liquid chromatography: A molecular perspective

Jake L. Rafferty, Ling Zhang, J. Ilja Siepmann, Mark R. Schure

Research output: Contribution to journalArticlepeer-review

137 Scopus citations

Abstract

A detailed, molecular-level understanding of the retention mechanism in reversed-phase liquid chromatography (RPLC) has eluded analytical chemists for decades. Through validated, particle-based Monte Carlo simulations of a model RPLC system consisting of dimethyloctadecylsilanes at a coverage of 2.9 μmol/m2 on an explicit silica substrate with unprotected residual silanols in contact with a water/methanol mobile phase, we show that the molecular-level retention processes for nonpolar and polar analytes, such as alkanes and alcohols, are much more complex than what has been previously deduced from thermodynamic and theoretical arguments. In contrast to some previous assumptions, the simulations indicate that both partitioning and adsorption play a key role in the separation process and that the stationary phase in RPLC behaves substantially different from a bulk hydrocarbon phase. The retention of nonpolar methylene segments is dominated by lipophilic interactions with the retentive phase, while solvophilic interactions are more important for the retention of the polar hydroxyl group.

Original languageEnglish (US)
Pages (from-to)6551-6558
Number of pages8
JournalAnalytical chemistry
Volume79
Issue number17
DOIs
StatePublished - Sep 1 2007

Fingerprint

Dive into the research topics of 'Retention mechanism in reversed-phase liquid chromatography: A molecular perspective'. Together they form a unique fingerprint.

Cite this