RET signaling is essential for migration, axonal growth and axon guidance of developing sympathetic neurons

H. Enomoto, P. A. Crawford, A. Gorodinsky, R. O. Heuckeroth, E. M. Johnson, J. Milbrandt

Research output: Contribution to journalArticle

218 Scopus citations

Abstract

Sympathetic axons use blood vessels as an intermediate path to reach their final target tissues. The initial contact between differentiating sympathetic neurons and blood vessels occurs following the primary sympathetic chain formation, where precursors of sympathetic neurons migrate and project axons along or toward blood vessels. We demonstrate that, in Ret-deficient mice, neuronal precursors throughout the entire sympathetic nervous system fail to migrate and project axons properly. These primary deficits lead to mis-routing of sympathetic nerve trunks and accelerated cell death of sympathetic neurons later in development. Artemin is expressed in blood vessels during periods of early sympathetic differentiation, and can promote and attract axonal growth of the sympathetic ganglion in vitro. This analysis identifies RET and artemin as central regulators of early sympathetic innervation.

Original languageEnglish (US)
Pages (from-to)3963-3974
Number of pages12
JournalDevelopment
Volume128
Issue number20
StatePublished - Nov 20 2001

Keywords

  • Axon growth
  • Axon guidance
  • Knockout mouse
  • Migration
  • Ret
  • Sympathetic neuron

Fingerprint Dive into the research topics of 'RET signaling is essential for migration, axonal growth and axon guidance of developing sympathetic neurons'. Together they form a unique fingerprint.

  • Cite this

    Enomoto, H., Crawford, P. A., Gorodinsky, A., Heuckeroth, R. O., Johnson, E. M., & Milbrandt, J. (2001). RET signaling is essential for migration, axonal growth and axon guidance of developing sympathetic neurons. Development, 128(20), 3963-3974.