Response of the hepatic transcriptome to aflatoxin b1in domestic turkey (Meleagris gallopavo)

Melissa S. Monson, Robert E. Settlage, Kevin W. McMahon, Kristelle M. Mendoza, Sumit Rawal, Hani S. El-Nezami, Roger A. Coulombe, Kent M. Reed

Research output: Contribution to journalArticlepeer-review

29 Scopus citations


Dietary exposure to aflatoxin B1 (AFB1) is detrimental to avian health and leads to major economic losses for the poultry industry. AFB1 is especially hepatotoxic in domestic turkeys (Meleagris gallopavo), since these birds are unable to detoxify AFB1 by glutathione-conjugation. The impacts of AFB1 on the turkey hepatic transcriptome and the potential protection from pretreatment with a Lactobacillus-based probiotic mixture were investigated through RNA-sequencing. Animals were divided into four treatment groups and RNA was subsequently recovered from liver samples. Four pooled RNA-seq libraries were sequenced to produce over 322 M reads totaling 13.8 Gb of sequence. Approximately 170,000 predicted transcripts were de novo assembled, of which 803 had significant differential expression in at least one pair-wise comparison between treatment groups. Functional analysis linked many of the transcripts significantly affected by AFB1 exposure to cancer, apoptosis, the cell cycle or lipid regulation. Most notable were transcripts from the genes encoding E3 ubiquitin-protein ligase Mdm2, osteopontin, S-adenosylmethionine synthase isoform type-2, and lipoprotein lipase. Expression was modulated by the probiotics, but treatment did not completely mitigate the effects of AFB 1. Genes identified through transcriptome analysis provide candidates for further study of AFB1 toxicity and targets for efforts to improve the health of domestic turkeys exposed to AFB1.

Original languageEnglish (US)
Article numbere100930
JournalPloS one
Issue number6
StatePublished - Jun 30 2014


Dive into the research topics of 'Response of the hepatic transcriptome to aflatoxin b1in domestic turkey (Meleagris gallopavo)'. Together they form a unique fingerprint.

Cite this