Response of the cell membrane-cytoskeleton complex to osmotic and freeze/thaw stresses. Part 2: The link between the state of the membrane-cytoskeleton complex and the cellular damage

Vishard Ragoonanan, Rebekah Less, Alptekin Aksan

Research output: Contribution to journalArticlepeer-review

15 Scopus citations

Abstract

In an earlier paper [35], we examined the mutual interaction between the actin cytoskeleton and the cell membrane and explored the role this interaction plays during freeze/thaw. In this follow-up paper, we investigate the physical and chemical stresses induced by freeze/thaw and explore the different mechanisms of damage caused by these stresses. Our results showed that changes in cell volume during freeze/thaw and the unfrozen water content in the solution alter the cytoskeleton stiffness, and the available membrane material. Combined with unfavorable ice-membrane interactions and increasing membrane stiffness, increased de-structuring of the membrane (such as bleb and microvilli formation) synergistically act on the membrane-cytoskeleton system generating irreversible damage.

Original languageEnglish (US)
Pages (from-to)96-104
Number of pages9
JournalCryobiology
Volume66
Issue number2
DOIs
StatePublished - Apr 2013

Bibliographical note

Funding Information:
Statement of funding: This research was partially supported by a University of Minnesota Doctoral Dissertation Fellowship awarded to V.R. and the University of Minnesota McKnight Land-Grant Professorship awarded to A.A. as well as an NSF Grant ( CBET-0644784 ) and its REU supplement.

Copyright:
Copyright 2013 Elsevier B.V., All rights reserved.

Keywords

  • Actin cytoskeleton
  • Cryopreservation
  • Freeze/thaw
  • Membrane

Fingerprint Dive into the research topics of 'Response of the cell membrane-cytoskeleton complex to osmotic and freeze/thaw stresses. Part 2: The link between the state of the membrane-cytoskeleton complex and the cellular damage'. Together they form a unique fingerprint.

Cite this