Abstract
Protein kinase CK2 is one of the key cellular signals for cell survival, growth, and proliferation. It is has been observed to be elevated in various cancers that have been examined. Various observations suggest that moderate dysregulation of CK2 may profoundly influence the cell response. We have examined the effects of interfering with the CK2 signal in various cancer cell lines by employing antisense oligodeoxynucleotides (ODN) against the α and β subunits of CK2. Our results demonstrate that antisense CK2-α and antisense CK2-β ODNs markedly influence cell viability of these cancer cells in a dose and time-dependent manner. Antisense CK2-α was slightly more effective than antisense CK2-β in most of the cells tested. The efficacy of the antisense ODN seemed to vary with the cell type; however, in all cases potent induction of apoptosis was observed. Significantly, the effects of the antisense ODN on the CK2 activity in the nuclear matrix were relatively small compared to the much stronger induction of apoptosis in cells. This suggests that modest downregulation of CK2 can evoke a much greater apoptotic response in cancer cells.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 167-174 |
| Number of pages | 8 |
| Journal | Molecular and cellular biochemistry |
| Volume | 227 |
| Issue number | 1-2 |
| DOIs | |
| State | Published - 2001 |
Bibliographical note
Funding Information:This work was supported in part by United States Public Health Service Research Grant CA-15062 awarded by the National Cancer Institute, Department of Health and Human Services, and in part by the Medical Research Fund of the United States Department of Veterans Affairs.
Keywords
- Antisense
- Gene therapy
- Head and neck cancer
- Nuclear matrix
- Prostate cancer
- Protein kinase CK2
