TY - JOUR
T1 - Respiratory syncytial virus infection of alveolar macrophages in adult transplant patients
AU - Panuska, J. R.
AU - Hertz, M. I.
AU - Taraf, H.
AU - Villani, A.
AU - Cirino, N. M.
PY - 1992
Y1 - 1992
N2 - Pulmonary epithelial cells are thought to be the primary cellular targets for infection by respiratory syncytial virus (RSV) in vivo. To determine whether other pulmonary cells are infected by RSV, bronchoalveolar lavage cells from six adult transplant patients, four of whom had acute RSV infection, were examined by in situ immunohistochemistry to identify infected lung cells. Both alveolar macrophages and epithelial cells were infected with RSV in vivo. At the single-cell level, three-color immunofluorescent studies revealed that both RSV-infected epithelial cells and alveolar macrophages expressed Class II molecules of the major histocompatibility complex, but only the alveolar macrophage coexpressed interleukin-1β. Paraformaldehyde- fixed bronchoalveolar lavage cells from RSV-infected but not uninfected patients induced a marked proliferative response by cloned T cells indicating that in vivo infected cells expressed bioactive interleukin-1. Together, these studies indicate that the alveolar macrophage may have a critical role in the lung immune response to RSV.
AB - Pulmonary epithelial cells are thought to be the primary cellular targets for infection by respiratory syncytial virus (RSV) in vivo. To determine whether other pulmonary cells are infected by RSV, bronchoalveolar lavage cells from six adult transplant patients, four of whom had acute RSV infection, were examined by in situ immunohistochemistry to identify infected lung cells. Both alveolar macrophages and epithelial cells were infected with RSV in vivo. At the single-cell level, three-color immunofluorescent studies revealed that both RSV-infected epithelial cells and alveolar macrophages expressed Class II molecules of the major histocompatibility complex, but only the alveolar macrophage coexpressed interleukin-1β. Paraformaldehyde- fixed bronchoalveolar lavage cells from RSV-infected but not uninfected patients induced a marked proliferative response by cloned T cells indicating that in vivo infected cells expressed bioactive interleukin-1. Together, these studies indicate that the alveolar macrophage may have a critical role in the lung immune response to RSV.
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U2 - 10.1164/ajrccm/145.4_pt_1.934
DO - 10.1164/ajrccm/145.4_pt_1.934
M3 - Article
C2 - 1554223
AN - SCOPUS:0026587418
SN - 0003-0805
VL - 145
SP - 934
EP - 939
JO - American Review of Respiratory Disease
JF - American Review of Respiratory Disease
IS - 4 I
ER -