Cellular proteins were not synthesized by germinating ascospores of Neurospora tetrasperma until 90 min after spore activation. Nevertheless, immediately after activation these ascospores developed a cyanide-sensitive respiration which increased throughout this 90-min period. At 90 min the respiratory rates accelerated rapidly, protein synthesis was initiated, and transcripts for a subunit of the mitochondrial ATPase, employed here as a representative mRNA, began to accumulate.
Bibliographical noteFunding Information:
This research was supported by grams from the National Institute of General Medical Sciences (GM-19398), the United States Department of Agriculture Competitive Research Grants Office (88-37264-4045L and the Bush Foundation.
- Cyanide-sensitive respiration
- Gene expression
- Mitochondrial ATPase
- Neurospora tetrasperma
- Spore germination