Resonance Raman Studies of Pyrocatechase-Inhibitor Complexes

Lawrence Que, Ruth Mayer, A. Roe Lawrence, Heistand Robert

Research output: Contribution to journalArticle

48 Scopus citations

Abstract

The resonance Raman spectra of native pyro-catechase and its benzoate and phenolate complexes were investigated by using the available lines of an argon and a krypton laser. The data provide evidence for the presence of two distinct tyrosines coordinated to the active-site iron. The two tyrosines exhibit different vco values which show maximum resonance enhancements at different excitation wavelengths. Moreover, one tyrosine is more susceptible to changes in the active-site environment. Pyrocatechase is the only example thus far among iron-tyrosinate proteins where the tyrosines coordinating the iron are distinguishable.

Original languageEnglish (US)
Pages (from-to)2588-2593
Number of pages6
JournalBiochemistry
Volume19
Issue number12
DOIs
StatePublished - Jan 1 1980

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