TY - JOUR
T1 - Resolution of racemic 2-chlorophenyl glycine with immobilized penicillin G acylase
AU - Fadnavis, Nitin W.
AU - Vedamayee Devi, Avala
AU - Swarnalatha Jasti, Lakshmi
N1 - Funding Information:
We thank the Council of Scientific and Industrial Research, New Delhi for financial support.
PY - 2008/10/20
Y1 - 2008/10/20
N2 - Racemic 2-chlorophenyl glycine has been resolved to obtain (S)-α-amino-(2-chlorophenyl)acetic acid with >99% enantiomeric purity via enantioselective hydrolysis of its N-phenylacetyl derivative with penicillin G acylase immobilized on Eupergit C. The resolution can be conveniently performed in water at substrate concentration of 10% in 5 h with an enzyme:substrate ratio of 1:5 (w/w), and the enzyme can be recycled >25 times without any loss of activity. The unwanted (R)-enantiomer can be easily racemized for recycling.
AB - Racemic 2-chlorophenyl glycine has been resolved to obtain (S)-α-amino-(2-chlorophenyl)acetic acid with >99% enantiomeric purity via enantioselective hydrolysis of its N-phenylacetyl derivative with penicillin G acylase immobilized on Eupergit C. The resolution can be conveniently performed in water at substrate concentration of 10% in 5 h with an enzyme:substrate ratio of 1:5 (w/w), and the enzyme can be recycled >25 times without any loss of activity. The unwanted (R)-enantiomer can be easily racemized for recycling.
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U2 - 10.1016/j.tetasy.2008.10.015
DO - 10.1016/j.tetasy.2008.10.015
M3 - Article
AN - SCOPUS:56949098025
SN - 0957-4166
VL - 19
SP - 2363
EP - 2366
JO - Tetrahedron Asymmetry
JF - Tetrahedron Asymmetry
IS - 20
ER -