TY - JOUR
T1 - Resistance traits and AFLP characterization of diploid primitive tuber-bearing potatoes
AU - Aversano, Riccardo
AU - Ercolano, Maria Raffaella
AU - Frusciante, Luigi
AU - Monti, Luigi
AU - Bradeen, James M.
AU - Cristinzio, Gennaro
AU - Zoina, Astolfo
AU - Greco, Nicola
AU - Vitale, Salvatore
AU - Carputo, Domenico
PY - 2007/12
Y1 - 2007/12
N2 - Worldwide, a variety of pathogens negatively affect potato production, resulting in an estimated 22% annual yield reduction. Wild Solanum species represent a unique gene pool where all the traits necessary to improve the cultivated potato can be found. Therefore, breeding efforts for improved disease resistance and research aimed at characterizing wild germplasm have been extensively made. In this paper, sources of resistance to Phytophthora infestans, Erwinia carotovora subsp. carotovora, Fusarium solani and Globodera spp. have been investigated in several clones of two Solanum species originating from Central Mexico (S. bulbocastanum and S. cardiophyllum). Interestingly, we found sources of combined resistance to late blight and bacterial soft rot. This is an important finding considering that the development of resistant potato varieties has been hindered by the scarcity of resistant germplasm. In addition, we explored molecular differences within and between the two species generating AFLP fingerprints. By means of six primer pair combinations, we found 13 and 16 putative species-specific AFLP markers for S. bulbocastanum and S. cardiophyllum, respectively, and a bounty of markers useful for mapping, MAS, and cloning purposes. The phenotypic and molecular information associated to S. bulbocastanum and S. cardiophyllum for designing strategies of assisted selection are discussed.
AB - Worldwide, a variety of pathogens negatively affect potato production, resulting in an estimated 22% annual yield reduction. Wild Solanum species represent a unique gene pool where all the traits necessary to improve the cultivated potato can be found. Therefore, breeding efforts for improved disease resistance and research aimed at characterizing wild germplasm have been extensively made. In this paper, sources of resistance to Phytophthora infestans, Erwinia carotovora subsp. carotovora, Fusarium solani and Globodera spp. have been investigated in several clones of two Solanum species originating from Central Mexico (S. bulbocastanum and S. cardiophyllum). Interestingly, we found sources of combined resistance to late blight and bacterial soft rot. This is an important finding considering that the development of resistant potato varieties has been hindered by the scarcity of resistant germplasm. In addition, we explored molecular differences within and between the two species generating AFLP fingerprints. By means of six primer pair combinations, we found 13 and 16 putative species-specific AFLP markers for S. bulbocastanum and S. cardiophyllum, respectively, and a bounty of markers useful for mapping, MAS, and cloning purposes. The phenotypic and molecular information associated to S. bulbocastanum and S. cardiophyllum for designing strategies of assisted selection are discussed.
KW - Germplasm
KW - Molecular markers
KW - S. cardiophyllum
KW - Solanum bulbocastanum
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U2 - 10.1007/s10722-006-9201-6
DO - 10.1007/s10722-006-9201-6
M3 - Article
AN - SCOPUS:36448961990
SN - 0925-9864
VL - 54
SP - 1797
EP - 1806
JO - Genetic Resources and Crop Evolution
JF - Genetic Resources and Crop Evolution
IS - 8
ER -