Resistance to stem rust Ug99 in six bread wheat cultivars maps to chromosome 6DS

Eric E. Lopez-Vera, Sarah Nelson, Ravi P. Singh, Bhoja R. Basnet, Scott D. Haley, Sridhar Bhavani, Julio Huerta-Espino, Beatriz G. Xoconostle-Cazares, Roberto Ruiz-Medrano, Matthew N Rouse, Sukhwinder Singh

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28 Scopus citations


Key message: Identified SSR markers (Xcfd49 and Xbarc183) linked with stem rust resistance for efficient use in marker-assisted selection and stacking of resistance genes in wheat breeding programs. More than 80 % of the worldwide wheat (Triticum aestivum L.) area is currently sown with varieties susceptible to the Ug99 race group of stem rust fungus. However, wheat lines Niini, Tinkio, Coni, Pfunye, Blouk, and Ripper have demonstrated Ug99 resistance at the seedling and adult plant stages. We mapped stem rust resistance in populations derived from crosses of a susceptible parent with each of the resistant lines. The segregation of resistance in each population indicated the presence of a single gene. The resistance gene in Niini mapped to short arm of chromosome 6D and was flanked by SSR markers Xcfd49 at distances of 3.9 cM proximal and Xbarc183 8.4 cM distal, respectively. The chromosome location of this resistance was validated in three other populations: PBW343/Coni, PBW343/Tinkio, and Cacuke/Pfunye. Resistance initially postulated to be conferred by the SrTmp gene in Blouk and Ripper was also linked to Xcfd49 and Xbarc183 on 6DS, but it was mapped proximal to Xbarc183 at a similar position to previously mapped genes Sr42 and SrCad. Based on the variation in diagnostic marker alleles, it is possible that Niini and Pfunye may carry different resistance genes/alleles. Further studies are needed to determine the allelic relationships between various genes located on chromosome arm 6DS. Our results provide valuable molecular marker and genetic information for developing Ug99 resistant wheat varieties in diverse germplasm and using these markers to tag the resistance genes in wheat breeding.

Original languageEnglish (US)
Pages (from-to)231-239
Number of pages9
JournalTheoretical and Applied Genetics
Issue number1
StatePublished - Jan 2014

Bibliographical note

Funding Information:
This research work was supported by the Durable Rust Resistance in Wheat (DRRW) project managed by Cornell University with the Bill & Melinda Gates Foundation and the UK Agency for International Development (DFID). E.E. Lopez-Vera appreciates the Ph.D. fellowship from Mexico’s Consejo Nacional de Ciencia y Tecnología (CONACYT). Editing assistance was received from E. Quilligan (CIMMYT).


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