Resistance of Staphylococcus aureus to penicillins and cephalosporins: reversal of intrinsic resistance with some chelating agents.

L. D. Sabath, S. J. Wallace, K. Byers, L. D. Sabath

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Methicillin resistance of 10 out of 11 strains of S. aureus could be selectively suppressed by a factor as great as >1024 in the presence of EDTA, though the susceptibility of methicillin susceptible (M S) strains of S. aureus was essentially unchanged. EDTA also sporadically, selectively reduced the number of phages that reacted with methicillin resistant (M R) strains of S. aureus, but had essentially no effect on the phage susceptibility of M S strains. The selective effect of EDTA on M R but not on M S strains could also be seen in relation to susceptibility to penicillin, cephaloridine, but not to most other antibiotics. Two other chelating agents, o phenanthroline and 8 hydroxyquinoline, were also tested: the former also had a selective detectable (but lesser) effect on the methicillin resistance of M R strains, but the latter was almost without effect. These results suggest that a metal (or metals) play a critical role in the 'intrinsic resistance' of S. aureus to penicillins and cephalosporins.
Original languageUndefined/Unknown
Pages (from-to)435-443
Number of pages9
JournalAnnals of the New York Academy of Sciences
Volume236
StatePublished - 1974

Keywords

  • drug resistance
  • microorganism
  • penicillin resistance
  • Staphylococcus aureus
  • theoretical study
  • 1,10 phenanthroline
  • 8 quinolinol
  • bacitracin
  • cefaloridine
  • cefalotin
  • cephalosporin
  • chloramphenicol
  • edetic acid
  • erythromycin
  • gentamicin
  • lincomycin
  • meticillin
  • penicillin G
  • streptomycin
  • tetracycline
  • vancomycin

Cite this

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title = "Resistance of Staphylococcus aureus to penicillins and cephalosporins: reversal of intrinsic resistance with some chelating agents.",
abstract = "Methicillin resistance of 10 out of 11 strains of S. aureus could be selectively suppressed by a factor as great as >1024 in the presence of EDTA, though the susceptibility of methicillin susceptible (M S) strains of S. aureus was essentially unchanged. EDTA also sporadically, selectively reduced the number of phages that reacted with methicillin resistant (M R) strains of S. aureus, but had essentially no effect on the phage susceptibility of M S strains. The selective effect of EDTA on M R but not on M S strains could also be seen in relation to susceptibility to penicillin, cephaloridine, but not to most other antibiotics. Two other chelating agents, o phenanthroline and 8 hydroxyquinoline, were also tested: the former also had a selective detectable (but lesser) effect on the methicillin resistance of M R strains, but the latter was almost without effect. These results suggest that a metal (or metals) play a critical role in the 'intrinsic resistance' of S. aureus to penicillins and cephalosporins.",
keywords = "drug resistance, microorganism, penicillin resistance, Staphylococcus aureus, theoretical study, 1,10 phenanthroline, 8 quinolinol, bacitracin, cefaloridine, cefalotin, cephalosporin, chloramphenicol, edetic acid, erythromycin, gentamicin, lincomycin, meticillin, penicillin G, streptomycin, tetracycline, vancomycin",
author = "Sabath, {L. D.} and Wallace, {S. J.} and K. Byers and Sabath, {L. D.}",
year = "1974",
language = "Undefined/Unknown",
volume = "236",
pages = "435--443",
journal = "Annals of the New York Academy of Sciences",
issn = "0077-8923",
publisher = "Wiley-Blackwell",

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TY - JOUR

T1 - Resistance of Staphylococcus aureus to penicillins and cephalosporins: reversal of intrinsic resistance with some chelating agents.

AU - Sabath, L. D.

AU - Wallace, S. J.

AU - Byers, K.

AU - Sabath, L. D.

PY - 1974

Y1 - 1974

N2 - Methicillin resistance of 10 out of 11 strains of S. aureus could be selectively suppressed by a factor as great as >1024 in the presence of EDTA, though the susceptibility of methicillin susceptible (M S) strains of S. aureus was essentially unchanged. EDTA also sporadically, selectively reduced the number of phages that reacted with methicillin resistant (M R) strains of S. aureus, but had essentially no effect on the phage susceptibility of M S strains. The selective effect of EDTA on M R but not on M S strains could also be seen in relation to susceptibility to penicillin, cephaloridine, but not to most other antibiotics. Two other chelating agents, o phenanthroline and 8 hydroxyquinoline, were also tested: the former also had a selective detectable (but lesser) effect on the methicillin resistance of M R strains, but the latter was almost without effect. These results suggest that a metal (or metals) play a critical role in the 'intrinsic resistance' of S. aureus to penicillins and cephalosporins.

AB - Methicillin resistance of 10 out of 11 strains of S. aureus could be selectively suppressed by a factor as great as >1024 in the presence of EDTA, though the susceptibility of methicillin susceptible (M S) strains of S. aureus was essentially unchanged. EDTA also sporadically, selectively reduced the number of phages that reacted with methicillin resistant (M R) strains of S. aureus, but had essentially no effect on the phage susceptibility of M S strains. The selective effect of EDTA on M R but not on M S strains could also be seen in relation to susceptibility to penicillin, cephaloridine, but not to most other antibiotics. Two other chelating agents, o phenanthroline and 8 hydroxyquinoline, were also tested: the former also had a selective detectable (but lesser) effect on the methicillin resistance of M R strains, but the latter was almost without effect. These results suggest that a metal (or metals) play a critical role in the 'intrinsic resistance' of S. aureus to penicillins and cephalosporins.

KW - drug resistance

KW - microorganism

KW - penicillin resistance

KW - Staphylococcus aureus

KW - theoretical study

KW - 1,10 phenanthroline

KW - 8 quinolinol

KW - bacitracin

KW - cefaloridine

KW - cefalotin

KW - cephalosporin

KW - chloramphenicol

KW - edetic acid

KW - erythromycin

KW - gentamicin

KW - lincomycin

KW - meticillin

KW - penicillin G

KW - streptomycin

KW - tetracycline

KW - vancomycin

M3 - Article

VL - 236

SP - 435

EP - 443

JO - Annals of the New York Academy of Sciences

JF - Annals of the New York Academy of Sciences

SN - 0077-8923

ER -