Resistance gene cloning from a wild crop relative by sequence capture and association genetics

Sanu Arora; Burkhard Steuernagel; Kumar Gaurav; Sutha Chandramohan; Yunming Long; Oadi Matny; Ryan Johnson; Jacob Enk; Sambasivam Periyannan; Narinder Singh; M. Asyraf Md Hatta; Naveenkumar Athiyannan; Jitender Cheema; Guotai Yu; Ngonidzashe Kangara; Sreya Ghosh; Les J. Szabo; Jesse Poland; Harbans Bariana; Jonathan D.G. Jones; Alison R. Bentley; Mick Ayliffe; Eric Olson; Steven S. Xu; Brian J. Steffenson; Evans Lagudah; Brande B.H. Wulff

doi:10.1038/s41587-018-0007-9

Resistance gene cloning from a wild crop relative by sequence capture and association genetics

Sanu Arora, Burkhard Steuernagel, Kumar Gaurav, Sutha Chandramohan, Yunming Long, Oadi Matny, Ryan Johnson, Jacob Enk, Sambasivam Periyannan, Narinder Singh, M. Asyraf Md Hatta, Naveenkumar Athiyannan, Jitender Cheema, Guotai Yu, Ngonidzashe Kangara, Sreya Ghosh, Les J. Szabo, Jesse Poland, Harbans Bariana, Jonathan D.G. JonesAlison R. Bentley, Mick Ayliffe, Eric Olson, Steven S. Xu, Brian J. Steffenson, Evans Lagudah, Brande B.H. Wulff

Plant Pathology

Research output: Contribution to journal › Article › peer-review

169 Scopus citations

Abstract

Disease resistance (R) genes from wild relatives could be used to engineer broad-spectrum resistance in domesticated crops. We combined association genetics with R gene enrichment sequencing (AgRenSeq) to exploit pan-genome variation in wild diploid wheat and rapidly clone four stem rust resistance genes. AgRenSeq enables R gene cloning in any crop that has a diverse germplasm panel.

Original language	English (US)
Pages (from-to)	139-143
Number of pages	5
Journal	Nature biotechnology
Volume	37
Issue number	2
DOIs	https://doi.org/10.1038/s41587-018-0007-9
State	Published - Feb 1 2019

Bibliographical note

Funding Information:
We are grateful to the germplasm banks at Kansas State University, International Center for Agricultural Research in the Dry Areas, USDA-ARS National Small Grains Collection, and the N. I. Vavilov Research Institute of Plant Industry, and to our colleagues J. Raupp, J. Dvorak, and C. Hiebert for providing seed of Ae. tauschii. We thank our colleagues Y. Yue and JIC Horticultural Services for plant husbandry, J. Brown for helpful discussions, M. Ambrose and A. Meldrum for help with MTAs, K. Witek for technical assistance with RenSeq, M. Rouse and Y. Jin for PGT isolates, P. Solomon for help with bait source sequences, the International Wheat Sequencing Consortium for pre-publication access to REFSEQ v1.0, and B. McIntosh for critical reading of the manuscript. This research was supported by the NBI Computing Infrastructure for Science (CiS) group and financed by the Two Blades Foundation, USA to B.J.S. and B.B.H.W.; the Biotechnology and Biological Sciences Research Council (BBSRC) to B.B.H.W.; the BBSRC Designing Future Wheat Cross-Institute Strategic Programme to A.R.B. and B.B.H.W.; the Norwich Research Park Translational Fund to B.B.H.W.; the Lieberman-Okinow Endowment at the University of Minnesota to B.J.S.; the Grains Research and Development Corporation, Australia to E.L. and H.B.; the Gordon and Betty Moore Foundation to J.D.G.J.; and in-kind support by Arbor Biosciences.

Publisher Copyright:
© 2019, The Author(s), under exclusive licence to Springer Nature America, Inc.

Publisher link

10.1038/s41587-018-0007-9

Find It

Find It at U of M Libraries

Cite this

Arora, S., Steuernagel, B., Gaurav, K., Chandramohan, S., Long, Y., Matny, O., Johnson, R., Enk, J., Periyannan, S., Singh, N., Asyraf Md Hatta, M., Athiyannan, N., Cheema, J., Yu, G., Kangara, N., Ghosh, S., Szabo, L. J., Poland, J., Bariana, H., ... Wulff, B. B. H. (2019). Resistance gene cloning from a wild crop relative by sequence capture and association genetics. Nature biotechnology, 37(2), 139-143. https://doi.org/10.1038/s41587-018-0007-9

Arora, S, Steuernagel, B, Gaurav, K, Chandramohan, S, Long, Y, Matny, O, Johnson, R, Enk, J, Periyannan, S, Singh, N, Asyraf Md Hatta, M, Athiyannan, N, Cheema, J, Yu, G, Kangara, N, Ghosh, S, Szabo, LJ, Poland, J, Bariana, H, Jones, JDG, Bentley, AR, Ayliffe, M, Olson, E, Xu, SS, Steffenson, BJ, Lagudah, E & Wulff, BBH 2019, 'Resistance gene cloning from a wild crop relative by sequence capture and association genetics', Nature biotechnology, vol. 37, no. 2, pp. 139-143. https://doi.org/10.1038/s41587-018-0007-9

@article{6c4da6b08192479096276118b986cd80,

title = "Resistance gene cloning from a wild crop relative by sequence capture and association genetics",

abstract = "Disease resistance (R) genes from wild relatives could be used to engineer broad-spectrum resistance in domesticated crops. We combined association genetics with R gene enrichment sequencing (AgRenSeq) to exploit pan-genome variation in wild diploid wheat and rapidly clone four stem rust resistance genes. AgRenSeq enables R gene cloning in any crop that has a diverse germplasm panel.",

author = "Sanu Arora and Burkhard Steuernagel and Kumar Gaurav and Sutha Chandramohan and Yunming Long and Oadi Matny and Ryan Johnson and Jacob Enk and Sambasivam Periyannan and Narinder Singh and {Asyraf Md Hatta}, M. and Naveenkumar Athiyannan and Jitender Cheema and Guotai Yu and Ngonidzashe Kangara and Sreya Ghosh and Szabo, {Les J.} and Jesse Poland and Harbans Bariana and Jones, {Jonathan D.G.} and Bentley, {Alison R.} and Mick Ayliffe and Eric Olson and Xu, {Steven S.} and Steffenson, {Brian J.} and Evans Lagudah and Wulff, {Brande B.H.}",

note = "Funding Information: We are grateful to the germplasm banks at Kansas State University, International Center for Agricultural Research in the Dry Areas, USDA-ARS National Small Grains Collection, and the N. I. Vavilov Research Institute of Plant Industry, and to our colleagues J. Raupp, J. Dvorak, and C. Hiebert for providing seed of Ae. tauschii. We thank our colleagues Y. Yue and JIC Horticultural Services for plant husbandry, J. Brown for helpful discussions, M. Ambrose and A. Meldrum for help with MTAs, K. Witek for technical assistance with RenSeq, M. Rouse and Y. Jin for PGT isolates, P. Solomon for help with bait source sequences, the International Wheat Sequencing Consortium for pre-publication access to REFSEQ v1.0, and B. McIntosh for critical reading of the manuscript. This research was supported by the NBI Computing Infrastructure for Science (CiS) group and financed by the Two Blades Foundation, USA to B.J.S. and B.B.H.W.; the Biotechnology and Biological Sciences Research Council (BBSRC) to B.B.H.W.; the BBSRC Designing Future Wheat Cross-Institute Strategic Programme to A.R.B. and B.B.H.W.; the Norwich Research Park Translational Fund to B.B.H.W.; the Lieberman-Okinow Endowment at the University of Minnesota to B.J.S.; the Grains Research and Development Corporation, Australia to E.L. and H.B.; the Gordon and Betty Moore Foundation to J.D.G.J.; and in-kind support by Arbor Biosciences. Publisher Copyright: {\textcopyright} 2019, The Author(s), under exclusive licence to Springer Nature America, Inc.",

year = "2019",

month = feb,

day = "1",

doi = "10.1038/s41587-018-0007-9",

language = "English (US)",

volume = "37",

pages = "139--143",

journal = "Biotechnology",

issn = "1087-0156",

publisher = "Nature Publishing Group",

number = "2",

}

TY - JOUR

T1 - Resistance gene cloning from a wild crop relative by sequence capture and association genetics

AU - Arora, Sanu

AU - Steuernagel, Burkhard

AU - Gaurav, Kumar

AU - Chandramohan, Sutha

AU - Long, Yunming

AU - Matny, Oadi

AU - Johnson, Ryan

AU - Enk, Jacob

AU - Periyannan, Sambasivam

AU - Singh, Narinder

AU - Asyraf Md Hatta, M.

AU - Athiyannan, Naveenkumar

AU - Cheema, Jitender

AU - Yu, Guotai

AU - Kangara, Ngonidzashe

AU - Ghosh, Sreya

AU - Szabo, Les J.

AU - Poland, Jesse

AU - Bariana, Harbans

AU - Jones, Jonathan D.G.

AU - Bentley, Alison R.

AU - Ayliffe, Mick

AU - Olson, Eric

AU - Xu, Steven S.

AU - Steffenson, Brian J.

AU - Lagudah, Evans

AU - Wulff, Brande B.H.

N1 - Funding Information: We are grateful to the germplasm banks at Kansas State University, International Center for Agricultural Research in the Dry Areas, USDA-ARS National Small Grains Collection, and the N. I. Vavilov Research Institute of Plant Industry, and to our colleagues J. Raupp, J. Dvorak, and C. Hiebert for providing seed of Ae. tauschii. We thank our colleagues Y. Yue and JIC Horticultural Services for plant husbandry, J. Brown for helpful discussions, M. Ambrose and A. Meldrum for help with MTAs, K. Witek for technical assistance with RenSeq, M. Rouse and Y. Jin for PGT isolates, P. Solomon for help with bait source sequences, the International Wheat Sequencing Consortium for pre-publication access to REFSEQ v1.0, and B. McIntosh for critical reading of the manuscript. This research was supported by the NBI Computing Infrastructure for Science (CiS) group and financed by the Two Blades Foundation, USA to B.J.S. and B.B.H.W.; the Biotechnology and Biological Sciences Research Council (BBSRC) to B.B.H.W.; the BBSRC Designing Future Wheat Cross-Institute Strategic Programme to A.R.B. and B.B.H.W.; the Norwich Research Park Translational Fund to B.B.H.W.; the Lieberman-Okinow Endowment at the University of Minnesota to B.J.S.; the Grains Research and Development Corporation, Australia to E.L. and H.B.; the Gordon and Betty Moore Foundation to J.D.G.J.; and in-kind support by Arbor Biosciences. Publisher Copyright: © 2019, The Author(s), under exclusive licence to Springer Nature America, Inc.

PY - 2019/2/1

Y1 - 2019/2/1

N2 - Disease resistance (R) genes from wild relatives could be used to engineer broad-spectrum resistance in domesticated crops. We combined association genetics with R gene enrichment sequencing (AgRenSeq) to exploit pan-genome variation in wild diploid wheat and rapidly clone four stem rust resistance genes. AgRenSeq enables R gene cloning in any crop that has a diverse germplasm panel.

AB - Disease resistance (R) genes from wild relatives could be used to engineer broad-spectrum resistance in domesticated crops. We combined association genetics with R gene enrichment sequencing (AgRenSeq) to exploit pan-genome variation in wild diploid wheat and rapidly clone four stem rust resistance genes. AgRenSeq enables R gene cloning in any crop that has a diverse germplasm panel.

UR - http://www.scopus.com/inward/record.url?scp=85061037610&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85061037610&partnerID=8YFLogxK

U2 - 10.1038/s41587-018-0007-9

DO - 10.1038/s41587-018-0007-9

M3 - Article

C2 - 30718880

AN - SCOPUS:85061037610

SN - 1087-0156

VL - 37

SP - 139

EP - 143

JO - Biotechnology

JF - Biotechnology

IS - 2

ER -

Resistance gene cloning from a wild crop relative by sequence capture and association genetics

Abstract

Bibliographical note

Publisher link

Find It

Other files and links

Fingerprint

Cite this