Resistance gene cloning from a wild crop relative by sequence capture and association genetics

Sanu Arora; Burkhard Steuernagel; Kumar Gaurav; Sutha Chandramohan; Yunming Long; Oadi Matny; Ryan Johnson; Jacob Enk; Sambasivam Periyannan; Narinder Singh; M. Asyraf Md Hatta; Naveenkumar Athiyannan; Jitender Cheema; Guotai Yu; Ngonidzashe Kangara; Sreya Ghosh; Les J. Szabo; Jesse Poland; Harbans Bariana; Jonathan D.G. Jones; Alison R. Bentley; Mick Ayliffe; Eric Olson; Steven S. Xu; Brian J. Steffenson; Evans Lagudah; Brande B.H. Wulff

doi:10.1038/s41587-018-0007-9

Resistance gene cloning from a wild crop relative by sequence capture and association genetics

Sanu Arora, Burkhard Steuernagel, Kumar Gaurav, Sutha Chandramohan, Yunming Long, Oadi Matny, Ryan Johnson, Jacob Enk, Sambasivam Periyannan, Narinder Singh, M. Asyraf Md Hatta, Naveenkumar Athiyannan, Jitender Cheema, Guotai Yu, Ngonidzashe Kangara, Sreya Ghosh, Les J. Szabo, Jesse Poland, Harbans Bariana, Jonathan D.G. JonesAlison R. Bentley, Mick Ayliffe, Eric Olson, Steven S. Xu, Brian J. Steffenson, Evans Lagudah, Brande B.H. Wulff

Plant Pathology

Research output: Contribution to journal › Article › peer-review

236 Scopus citations

Abstract

Disease resistance (R) genes from wild relatives could be used to engineer broad-spectrum resistance in domesticated crops. We combined association genetics with R gene enrichment sequencing (AgRenSeq) to exploit pan-genome variation in wild diploid wheat and rapidly clone four stem rust resistance genes. AgRenSeq enables R gene cloning in any crop that has a diverse germplasm panel.

Original language	English (US)
Pages (from-to)	139-143
Number of pages	5
Journal	Nature biotechnology
Volume	37
Issue number	2
DOIs	https://doi.org/10.1038/s41587-018-0007-9
State	Published - Feb 1 2019

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Publisher Copyright:
© 2019, The Author(s), under exclusive licence to Springer Nature America, Inc.

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10.1038/s41587-018-0007-9

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Arora, S., Steuernagel, B., Gaurav, K., Chandramohan, S., Long, Y., Matny, O., Johnson, R., Enk, J., Periyannan, S., Singh, N., Asyraf Md Hatta, M., Athiyannan, N., Cheema, J., Yu, G., Kangara, N., Ghosh, S., Szabo, L. J., Poland, J., Bariana, H., ... Wulff, B. B. H. (2019). Resistance gene cloning from a wild crop relative by sequence capture and association genetics. Nature biotechnology, 37(2), 139-143. https://doi.org/10.1038/s41587-018-0007-9

Arora, S, Steuernagel, B, Gaurav, K, Chandramohan, S, Long, Y, Matny, O, Johnson, R, Enk, J, Periyannan, S, Singh, N, Asyraf Md Hatta, M, Athiyannan, N, Cheema, J, Yu, G, Kangara, N, Ghosh, S, Szabo, LJ, Poland, J, Bariana, H, Jones, JDG, Bentley, AR, Ayliffe, M, Olson, E, Xu, SS, Steffenson, BJ, Lagudah, E & Wulff, BBH 2019, 'Resistance gene cloning from a wild crop relative by sequence capture and association genetics', Nature biotechnology, vol. 37, no. 2, pp. 139-143. https://doi.org/10.1038/s41587-018-0007-9

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abstract = "Disease resistance (R) genes from wild relatives could be used to engineer broad-spectrum resistance in domesticated crops. We combined association genetics with R gene enrichment sequencing (AgRenSeq) to exploit pan-genome variation in wild diploid wheat and rapidly clone four stem rust resistance genes. AgRenSeq enables R gene cloning in any crop that has a diverse germplasm panel.",

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AU - Arora, Sanu

AU - Steuernagel, Burkhard

AU - Gaurav, Kumar

AU - Chandramohan, Sutha

AU - Long, Yunming

AU - Matny, Oadi

AU - Johnson, Ryan

AU - Enk, Jacob

AU - Periyannan, Sambasivam

AU - Singh, Narinder

AU - Asyraf Md Hatta, M.

AU - Athiyannan, Naveenkumar

AU - Cheema, Jitender

AU - Yu, Guotai

AU - Kangara, Ngonidzashe

AU - Ghosh, Sreya

AU - Szabo, Les J.

AU - Poland, Jesse

AU - Bariana, Harbans

AU - Jones, Jonathan D.G.

AU - Bentley, Alison R.

AU - Ayliffe, Mick

AU - Olson, Eric

AU - Xu, Steven S.

AU - Steffenson, Brian J.

AU - Lagudah, Evans

AU - Wulff, Brande B.H.

PY - 2019/2/1

Y1 - 2019/2/1

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AB - Disease resistance (R) genes from wild relatives could be used to engineer broad-spectrum resistance in domesticated crops. We combined association genetics with R gene enrichment sequencing (AgRenSeq) to exploit pan-genome variation in wild diploid wheat and rapidly clone four stem rust resistance genes. AgRenSeq enables R gene cloning in any crop that has a diverse germplasm panel.

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Resistance gene cloning from a wild crop relative by sequence capture and association genetics

Abstract

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