Resistance gene cloning from a wild crop relative by sequence capture and association genetics

Sanu Arora, Burkhard Steuernagel, Kumar Gaurav, Sutha Chandramohan, Yunming Long, Oadi Matny, Ryan Johnson, Jacob Enk, Sambasivam Periyannan, Narinder Singh, M. Asyraf Md Hatta, Naveenkumar Athiyannan, Jitender Cheema, Guotai Yu, Ngonidzashe Kangara, Sreya Ghosh, Les J. Szabo, Jesse Poland, Harbans Bariana, Jonathan D.G. JonesAlison R. Bentley, Mick Ayliffe, Eric Olson, Steven S. Xu, Brian J. Steffenson, Evans Lagudah, Brande B.H. Wulff

Research output: Contribution to journalArticlepeer-review

169 Scopus citations


Disease resistance (R) genes from wild relatives could be used to engineer broad-spectrum resistance in domesticated crops. We combined association genetics with R gene enrichment sequencing (AgRenSeq) to exploit pan-genome variation in wild diploid wheat and rapidly clone four stem rust resistance genes. AgRenSeq enables R gene cloning in any crop that has a diverse germplasm panel.

Original languageEnglish (US)
Pages (from-to)139-143
Number of pages5
JournalNature biotechnology
Issue number2
StatePublished - Feb 1 2019

Bibliographical note

Funding Information:
We are grateful to the germplasm banks at Kansas State University, International Center for Agricultural Research in the Dry Areas, USDA-ARS National Small Grains Collection, and the N. I. Vavilov Research Institute of Plant Industry, and to our colleagues J. Raupp, J. Dvorak, and C. Hiebert for providing seed of Ae. tauschii. We thank our colleagues Y. Yue and JIC Horticultural Services for plant husbandry, J. Brown for helpful discussions, M. Ambrose and A. Meldrum for help with MTAs, K. Witek for technical assistance with RenSeq, M. Rouse and Y. Jin for PGT isolates, P. Solomon for help with bait source sequences, the International Wheat Sequencing Consortium for pre-publication access to REFSEQ v1.0, and B. McIntosh for critical reading of the manuscript. This research was supported by the NBI Computing Infrastructure for Science (CiS) group and financed by the Two Blades Foundation, USA to B.J.S. and B.B.H.W.; the Biotechnology and Biological Sciences Research Council (BBSRC) to B.B.H.W.; the BBSRC Designing Future Wheat Cross-Institute Strategic Programme to A.R.B. and B.B.H.W.; the Norwich Research Park Translational Fund to B.B.H.W.; the Lieberman-Okinow Endowment at the University of Minnesota to B.J.S.; the Grains Research and Development Corporation, Australia to E.L. and H.B.; the Gordon and Betty Moore Foundation to J.D.G.J.; and in-kind support by Arbor Biosciences.

Publisher Copyright:
© 2019, The Author(s), under exclusive licence to Springer Nature America, Inc.


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