Requirement of a centrosomal activity for cell cycle progression through G1 into S phase

E. H. Hinchcliffe, F. J. Miller, M. Cham, A. Khodjakov, G. Sluder

Research output: Contribution to journalArticle

293 Scopus citations

Abstract

Centrosomes were microsurgically removed from BSC-1 African green monkey kidney cells before the completion of S phase. Karyoplasts (acentrosomal cells) entered and completed mitosis. However, postmitotic karyoplasts arrested before S phase, whereas adjacent control cells divided repeatedly. Postmitotic karyoplasts assembled a microtubule-organizing center containing γ-tubulin and pericentrin, but did not regenerate centrioles. These observations reveal the existence of an activity associated with core centrosomal structures - distinct from elements of the microtubule-organizing center - that is required for the somatic cell cycle to progress through G1 into S phase. Once the cell is in S phase, these core structures are not needed for the G2-M phase transition.

Original languageEnglish (US)
Pages (from-to)1547-1550
Number of pages4
JournalScience
Volume291
Issue number5508
DOIs
StatePublished - Feb 23 2001
Externally publishedYes

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