Reproducibility of endothelial microparticles in children and adolescents

Elise F. Northrop, Liming C. Milbauer, Kyle D. Rudser, Claudia K. Fox, Anna N. Solovey, Alexander M. Kaizer, Robert P. Hebbel, Aaron S. Kelly, Justin R. Ryder

Research output: Contribution to journalArticle

Abstract

Aim: We assessed reproducibility of endothelial microparticles (EMPs) enumeration among youth. Methods & results: Four microparticle (MP) indices - total MP per microliter platelet free plasma (PFP), total EMPs per microliter PFP, percent activated EMPs and percent lactadherin positive (LACT[+]) of total EMPs - were measured at two visits (baseline and 7 ± 3 days follow-up) to determine reproducibility overall and by obesity status. We examined CD31+ or CD144+ with CD41-EMP events of size 0.3-1.0 μm. No statistically significant differences were observed between visits for any of the four MP indices. The within-participant and between-participant coefficient of variation was acceptable (range: 1.13-2.37) with good intraclass-correlation coefficient for all indices except total MP per microliter (range: 0.10-1.00). Conclusion: Total EMPs per microliter PFP, percent-activated EMPs and percent LACT(+) of total EMPs are reproducible among youth.

Original languageEnglish (US)
Pages (from-to)43-51
Number of pages9
JournalBiomarkers in Medicine
Volume14
Issue number1
DOIs
StatePublished - Jan 1 2019

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Keywords

  • biomarkers
  • endothelial
  • microparticles
  • obesity
  • reproducibility
  • youth

PubMed: MeSH publication types

  • Journal Article

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