Reproducibility of circulating endothelial cell enumeration and activation in children and adolescents

Justin R Ryder, Michael J. O'Connell, Kyle Rudser, Claudia K Fox, Anna Solovey, Robert P Hebbel, Aaron S Kelly

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Introduction: We examined the reproducibility of circulating endothelial cells (CEC) enumeration and activation among youth. Materials and methods: CECs from 151 youth were measured at baseline and 1 week follow-up. Enumeration of CEC in fresh whole blood was determined by direct assessment of buffy coat smears (CD146+ nucleated cells) and activated CEC (%VCAM-1 expression) was determined after immunomagnetic enrichment and co-staining of nuclei, plus positivity for P1H12 and VCAM-1. Results: No statistically significant difference in CEC enumeration (1.2 ± 2.5 vs 1.3 ± 2.2 CEC/milliliter of whole blood, p = 0.745) or activated CEC (57.1 ± 24.4 vs 58.0 ± 21.3 %VCAM-1, p = 0.592) between baseline and 1 week follow-up. Conclusion: On a cohort basis, CEC enumeration and activation are reproducible in youth. Relatively high individual biological variability may limit its clinical utility.

Original languageEnglish (US)
Pages (from-to)463-471
Number of pages9
JournalBiomarkers in Medicine
Volume10
Issue number5
DOIs
StatePublished - May 2016

Fingerprint

Endothelial cells
Endothelial Cells
Chemical activation
Vascular Cell Adhesion Molecule-1
Blood
Staining and Labeling

Keywords

  • biomarkers
  • cardiovascular disease risk
  • circulating endothelial cells
  • obesity
  • pediatric
  • reproducibility
  • youth

Cite this

Reproducibility of circulating endothelial cell enumeration and activation in children and adolescents. / Ryder, Justin R; O'Connell, Michael J.; Rudser, Kyle; Fox, Claudia K; Solovey, Anna; Hebbel, Robert P; Kelly, Aaron S.

In: Biomarkers in Medicine, Vol. 10, No. 5, 05.2016, p. 463-471.

Research output: Contribution to journalArticle

@article{c9d19736952d40068aa9080876b54f15,
title = "Reproducibility of circulating endothelial cell enumeration and activation in children and adolescents",
abstract = "Introduction: We examined the reproducibility of circulating endothelial cells (CEC) enumeration and activation among youth. Materials and methods: CECs from 151 youth were measured at baseline and 1 week follow-up. Enumeration of CEC in fresh whole blood was determined by direct assessment of buffy coat smears (CD146+ nucleated cells) and activated CEC ({\%}VCAM-1 expression) was determined after immunomagnetic enrichment and co-staining of nuclei, plus positivity for P1H12 and VCAM-1. Results: No statistically significant difference in CEC enumeration (1.2 ± 2.5 vs 1.3 ± 2.2 CEC/milliliter of whole blood, p = 0.745) or activated CEC (57.1 ± 24.4 vs 58.0 ± 21.3 {\%}VCAM-1, p = 0.592) between baseline and 1 week follow-up. Conclusion: On a cohort basis, CEC enumeration and activation are reproducible in youth. Relatively high individual biological variability may limit its clinical utility.",
keywords = "biomarkers, cardiovascular disease risk, circulating endothelial cells, obesity, pediatric, reproducibility, youth",
author = "Ryder, {Justin R} and O'Connell, {Michael J.} and Kyle Rudser and Fox, {Claudia K} and Anna Solovey and Hebbel, {Robert P} and Kelly, {Aaron S}",
year = "2016",
month = "5",
doi = "10.2217/bmm-2015-0051",
language = "English (US)",
volume = "10",
pages = "463--471",
journal = "Biomarkers in Medicine",
issn = "1752-0363",
publisher = "Future Medicine Ltd.",
number = "5",

}

TY - JOUR

T1 - Reproducibility of circulating endothelial cell enumeration and activation in children and adolescents

AU - Ryder, Justin R

AU - O'Connell, Michael J.

AU - Rudser, Kyle

AU - Fox, Claudia K

AU - Solovey, Anna

AU - Hebbel, Robert P

AU - Kelly, Aaron S

PY - 2016/5

Y1 - 2016/5

N2 - Introduction: We examined the reproducibility of circulating endothelial cells (CEC) enumeration and activation among youth. Materials and methods: CECs from 151 youth were measured at baseline and 1 week follow-up. Enumeration of CEC in fresh whole blood was determined by direct assessment of buffy coat smears (CD146+ nucleated cells) and activated CEC (%VCAM-1 expression) was determined after immunomagnetic enrichment and co-staining of nuclei, plus positivity for P1H12 and VCAM-1. Results: No statistically significant difference in CEC enumeration (1.2 ± 2.5 vs 1.3 ± 2.2 CEC/milliliter of whole blood, p = 0.745) or activated CEC (57.1 ± 24.4 vs 58.0 ± 21.3 %VCAM-1, p = 0.592) between baseline and 1 week follow-up. Conclusion: On a cohort basis, CEC enumeration and activation are reproducible in youth. Relatively high individual biological variability may limit its clinical utility.

AB - Introduction: We examined the reproducibility of circulating endothelial cells (CEC) enumeration and activation among youth. Materials and methods: CECs from 151 youth were measured at baseline and 1 week follow-up. Enumeration of CEC in fresh whole blood was determined by direct assessment of buffy coat smears (CD146+ nucleated cells) and activated CEC (%VCAM-1 expression) was determined after immunomagnetic enrichment and co-staining of nuclei, plus positivity for P1H12 and VCAM-1. Results: No statistically significant difference in CEC enumeration (1.2 ± 2.5 vs 1.3 ± 2.2 CEC/milliliter of whole blood, p = 0.745) or activated CEC (57.1 ± 24.4 vs 58.0 ± 21.3 %VCAM-1, p = 0.592) between baseline and 1 week follow-up. Conclusion: On a cohort basis, CEC enumeration and activation are reproducible in youth. Relatively high individual biological variability may limit its clinical utility.

KW - biomarkers

KW - cardiovascular disease risk

KW - circulating endothelial cells

KW - obesity

KW - pediatric

KW - reproducibility

KW - youth

UR - http://www.scopus.com/inward/record.url?scp=84969219557&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84969219557&partnerID=8YFLogxK

U2 - 10.2217/bmm-2015-0051

DO - 10.2217/bmm-2015-0051

M3 - Article

C2 - 27071934

AN - SCOPUS:84969219557

VL - 10

SP - 463

EP - 471

JO - Biomarkers in Medicine

JF - Biomarkers in Medicine

SN - 1752-0363

IS - 5

ER -