Reproducibility of circulating endothelial cell enumeration and activation in children and adolescents

Justin R Ryder, Michael J. O'Connell, Kyle Rudser, Claudia K Fox, Anna Solovey, Robert P Hebbel, Aaron S Kelly

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

Introduction: We examined the reproducibility of circulating endothelial cells (CEC) enumeration and activation among youth. Materials and methods: CECs from 151 youth were measured at baseline and 1 week follow-up. Enumeration of CEC in fresh whole blood was determined by direct assessment of buffy coat smears (CD146+ nucleated cells) and activated CEC (%VCAM-1 expression) was determined after immunomagnetic enrichment and co-staining of nuclei, plus positivity for P1H12 and VCAM-1. Results: No statistically significant difference in CEC enumeration (1.2 ± 2.5 vs 1.3 ± 2.2 CEC/milliliter of whole blood, p = 0.745) or activated CEC (57.1 ± 24.4 vs 58.0 ± 21.3 %VCAM-1, p = 0.592) between baseline and 1 week follow-up. Conclusion: On a cohort basis, CEC enumeration and activation are reproducible in youth. Relatively high individual biological variability may limit its clinical utility.

Original languageEnglish (US)
Pages (from-to)463-471
Number of pages9
JournalBiomarkers in Medicine
Volume10
Issue number5
DOIs
StatePublished - May 2016

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Keywords

  • biomarkers
  • cardiovascular disease risk
  • circulating endothelial cells
  • obesity
  • pediatric
  • reproducibility
  • youth

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