Reproducibility and agreement in evaluating retinal nerve fibre layer thickness between Stratus and Spectralis OCT

S. N. Arthur, S. D. Smith, M. M. Wright, A. L. Grajewski, Q. Wang, J. M. Terry, M. S. Lee

Research output: Contribution to journalArticlepeer-review

38 Scopus citations


Purpose To evaluate intra-device reproducibility of retinal nerve fibre layer (RNFL) measurements obtained using Stratus and Spectralis optical coherence tomography, and to analyze inter-device correlation and agreement for these measurements. Design Prospective observational study. Methods A total of 30 normal individuals participated in the study. One eye of each participant was scanned three times during one session by the same operator using Spectralis and Stratus. Intra-class correlation coefficients (ICCs), correlation coefficients (R), and Bland-Altman plots (BAPs) were used to assess reproducibility, correlation, and agreement between the two devices, respectively. Results A significant difference in mean RNFL thickness was seen between Stratus and Spectralis (106.2±6.9 μm vs 100.0±7.3 μm, P=0.0001). ICCs of RNFL thickness measurements ranged from 0.69 (clock hour 2; 95% confidence interval (95% CI): 0.54, 0.85) to 0.91 (inferior quadrant; 95% CI: 0.86, 0.96) for Stratus and were higher for Spectralis, ranging from 0.87 (temporal-superior sector; 95% CI: 0.79, 0.94) to 0.96 (global and nasal-inferior sector; 95% CI: 0.94, 0.99). Rs of RNFL thickness measurements between the two instruments ranged from 0.61 (temporal quadrant) to 0.87 (superior quadrant). BAPs demonstrated a systematic difference in RNFL values between the two devices, with Spectralis producing thinner RNFL values than Stratus. Conclusions Spectralis demonstrated higher ICCs and thinner RNFL measurements than Stratus. Although the inter-device correlation was good, differences in RNFL measurements obtained by the two devices indicate that these measurements would not be interchangeable in clinical evaluations.

Original languageEnglish (US)
Pages (from-to)192-200
Number of pages9
Issue number2
StatePublished - Feb 2011

Bibliographical note

Funding Information:
This study was supported, in part, by unrestricted grant from Research to Prevent Blindness Inc., New York, NY (MSL, MMW, ALG, JMT).


  • OCT and RNFL
  • Spectralis
  • Stratus
  • agreement
  • reproducibility


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