Replication of the broad-host-range plasmid RK2: isolation and characterization of a spontaneous deletion mutant that can replicate in Agrobacterium tumefaciens but not in Escherichia coli

Anath Das, Yong Hong Xie

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7 Scopus citations

Abstract

Two spontaneous deletions of a derivative of the broad-host-range plasmid RK2 were isolated from Agrobacterium tumefaciens. The two deletions have lost 56 and 505 bp, respectively, near the origin of replication (oriV). Of the eight 17-bp repeats present in the RK2 oriV, the smaller deletion has lost the first two while the larger one has lost the first three. The deletions led to a significant increase (3- to 7-fold) in plasmid copy number in A. tumefaciens, indicating their importance in copy number control. While the smaller deletion could replicate in Escherichia coli, the larger one could not. The role of the oriV sequences in the replication of pRK2 in A. tumefaciens and in E. coli is discussed.

Original languageEnglish (US)
Pages (from-to)309-315
Number of pages7
JournalMGG Molecular & General Genetics
Volume246
Issue number3
DOIs
StatePublished - May 1 1995

Keywords

  • Agrobacterium tumefaciens
  • Copy number
  • Host range
  • Replication

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