Clones from two Heliothis zea (Lepidoptera: Noctuidae) ovarian cell lines, BCIRL-Hz-AM1 (AM1) and BCIRL-Hz-AM3 (AM3), were generated and their ability to produce H. zea nuclear polyhedrosis virus (HzSNPV) was compared. Titers of extracellular virus (ECV) ranged from 5.5 (AM3-F9) to 44.9 × 105 PFU/ml (AM1-A4), with the parental cell lines AM3 and AM1 producing 14.8 and 26.4 × 105 PFU/ml, respectively. Concentrations of polyhedral inclusion bodies (PIB) produced by the cloned lines ranged from 0.7 (AM3-F9) to 59.6 × 106 PIB/ml (AM1-B3), while the parental cell lines generated 6.5 (AM3) and 12.9 × 106 PIB/ml (AM1). The percentage of cells from the cloned lines that produced PIB ranged from 39 to 86.4% for AM3-F9 and AM1-A7, respectively, with the parental lines exhibiting 49.1% (AM3) and 75.3% (AM1) cells with PIB. The number of PIB per cell also differed markedly between cell lines, varying from 18.3 (AM3-F9) to 184.4 (AM1-B3) PIB/cell. The parental lines produced 57.3 (AM3) and 75.9 (AM1) PIB/cell. Thus, significant differences were seen in virus production (ECV, PIB) between parental cell lines, as well as between parental cell lines and their clones. In addition, cell lines were characterized with regard to their growth rates and isoenzyme patterns.
Bibliographical noteFunding Information:
for his technical assistance, as F. Hink. C. Ignoffo, and S. Sohi for their review of this manuscript. This study supported by a U.S. Department of Agriculture, ricultural Research Service Postdoctoral Research sociateship.
Copyright 2014 Elsevier B.V., All rights reserved.
- Heliothis zea
- cell lines
- nuclear polyhedrosis virus
- virus replication