Replicating Large Genomes: Divide and Conquer

Juan Carlos Rivera-Mulia, David M. Gilbert

Research output: Contribution to journalReview articlepeer-review

51 Scopus citations


Complete duplication of large metazoan chromosomes requires thousands of potential initiation sites, only a small fraction of which are selected in each cell cycle. Assembly of the replication machinery is highly conserved and tightly regulated during the cell cycle, but the sites of initiation are highly flexible, and their temporal order of firing is regulated at the level of large-scale multi-replicon domains. Importantly, the number of replication forks must be quickly adjusted in response to replication stress to prevent genome instability. Here we argue that large genomes are divided into domains for exactly this reason. Once established, domain structure abrogates the need for precise initiation sites and creates a scaffold for the evolution of other chromosome functions.

Original languageEnglish (US)
Pages (from-to)756-765
Number of pages10
JournalMolecular Cell
Issue number5
StatePublished - Jun 2 2016
Externally publishedYes

Bibliographical note

Funding Information:
This work was supported by NIH grants GM083337 and GM085354 (D.M.G.).

Publisher Copyright:
© 2016 Elsevier Inc.


Dive into the research topics of 'Replicating Large Genomes: Divide and Conquer'. Together they form a unique fingerprint.

Cite this