Replacement of ParC α4 Helix with That of GyrA Increases the Stability and Cytotoxicity of Topoisomerase IV-Quinolone-DNA Ternary Complexes

Emily S. Pfeiffer; Hiroshi Hiasa

doi:10.1128/AAC.48.2.608-611.2004

Replacement of ParC α4 Helix with That of GyrA Increases the Stability and Cytotoxicity of Topoisomerase IV-Quinolone-DNA Ternary Complexes

Emily S. Pfeiffer, Hiroshi Hiasa

Pharmacology

Research output: Contribution to journal › Article › peer-review

24 Scopus citations

Abstract

Replacement of the α4 helix of ParC with that of GyrA increased the stability of topoisomerase IV-quinolone-DNA ternary complexes. This mutant topoisomerase IV-mediated cell killing was more efficient than topoisomerase IV-mediated cell killing in Escherichia coli. Thus, the α4 helix plays critical roles in determining the stability and the cytotoxicity of ternary complexes.

Original language	English (US)
Pages (from-to)	608-611
Number of pages	4
Journal	Antimicrobial agents and chemotherapy
Volume	48
Issue number	2
DOIs	https://doi.org/10.1128/AAC.48.2.608-611.2004
State	Published - Feb 2004

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abstract = "Replacement of the α4 helix of ParC with that of GyrA increased the stability of topoisomerase IV-quinolone-DNA ternary complexes. This mutant topoisomerase IV-mediated cell killing was more efficient than topoisomerase IV-mediated cell killing in Escherichia coli. Thus, the α4 helix plays critical roles in determining the stability and the cytotoxicity of ternary complexes.",

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Replacement of ParC α4 Helix with That of GyrA Increases the Stability and Cytotoxicity of Topoisomerase IV-Quinolone-DNA Ternary Complexes

Abstract

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