Repeated dose (28-day) administration of silver nanoparticles of varied size and coating does not significantly alter the indigenous murine gut microbiome

Laura A. Wilding, Christine M. Bassis, Kim Walacavage, Sara Hashway, Pascale R. Leroueil, Masako Morishita, Andrew D. Maynard, Martin A. Philbert, Ingrid L. Bergin

Research output: Contribution to journalArticle

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Abstract

Silver nanoparticles (AgNPs) have been used as antimicrobials in a number of applications, including topical wound dressings and coatings for consumer products and biomedical devices. Ingestion is a relevant route of exposure for AgNPs, whether occurring unintentionally via Ag dissolution from consumer products, or intentionally from dietary supplements. AgNP have also been proposed as substitutes for antibiotics in animal feeds. While oral antibiotics are known to have significant effects on gut bacteria, the antimicrobial effects of ingested AgNPs on the indigenous microbiome or on gut pathogens are unknown. In addition, AgNP size and coating have been postulated as significantly influential towards their biochemical properties and the influence of these properties on antimicrobial efficacy is unknown. We evaluated murine gut microbial communities using culture-independent sequencing of 16S rRNA gene fragments following 28 days of repeated oral dosing of well-characterized AgNPs of two different sizes (20 and 110 nm) and coatings (PVP and Citrate). Irrespective of size or coating, oral administration of AgNPs at 10 mg/kg body weight/day did not alter the membership, structure or diversity of the murine gut microbiome. Thus, in contrast to effects of broad-spectrum antibiotics, repeat dosing of AgNP, at doses equivalent to 2000 times the oral reference dose and 100-400 times the effective in vitro anti-microbial concentration, does not affect the indigenous murine gut microbiome.

LanguageEnglish (US)
Pages513-520
Number of pages8
JournalNanotoxicology
Volume10
Issue number5
DOIs
StatePublished - May 27 2016

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Silver
Nanoparticles
Antibiotics
Anti-Bacterial Agents
Coatings
Consumer products
Dietary supplements
Microbiota
Bandages
Dietary Supplements
rRNA Genes
Citric Acid
Oral Administration
Pathogens
Eating
Body Weight
Cell culture
Bacteria
Equipment and Supplies
Dissolution

Keywords

  • Antibiotics
  • in vivo
  • metastats
  • microbiome
  • mothur
  • mouse
  • nanomaterials
  • pyrosequencing
  • toxicology

Cite this

Repeated dose (28-day) administration of silver nanoparticles of varied size and coating does not significantly alter the indigenous murine gut microbiome. / Wilding, Laura A.; Bassis, Christine M.; Walacavage, Kim; Hashway, Sara; Leroueil, Pascale R.; Morishita, Masako; Maynard, Andrew D.; Philbert, Martin A.; Bergin, Ingrid L.

In: Nanotoxicology, Vol. 10, No. 5, 27.05.2016, p. 513-520.

Research output: Contribution to journalArticle

Wilding, LA, Bassis, CM, Walacavage, K, Hashway, S, Leroueil, PR, Morishita, M, Maynard, AD, Philbert, MA & Bergin, IL 2016, 'Repeated dose (28-day) administration of silver nanoparticles of varied size and coating does not significantly alter the indigenous murine gut microbiome', Nanotoxicology, vol. 10, no. 5, pp. 513-520. https://doi.org/10.3109/17435390.2015.1078854
Wilding, Laura A. ; Bassis, Christine M. ; Walacavage, Kim ; Hashway, Sara ; Leroueil, Pascale R. ; Morishita, Masako ; Maynard, Andrew D. ; Philbert, Martin A. ; Bergin, Ingrid L. / Repeated dose (28-day) administration of silver nanoparticles of varied size and coating does not significantly alter the indigenous murine gut microbiome. In: Nanotoxicology. 2016 ; Vol. 10, No. 5. pp. 513-520.
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