Repair of lesions which cause premature termination of transcription in chicken embryo cells irradiated with ultraviolet light

Perry B. Hackett, Harold E. Varmus, J. Michael Bishop

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

The dose-response curves for the synthesis of rRNA and avian sarcoma virus mRNA, after irradiation of virus-infected chicken embryo fibrolast cells, have shoulders (P. B. Hackett, H. E. Varmus, and J. M. Bishop, 1981. Virology, 112, 714-728). We tested the hypothesis that the shoulders were the result of dark repair of transcription-terminating lesions after irradiation by measuring the amounts of rRNA synthesized at various times after the uv dose was applied. Our data support the hypothesis and indicated that the rate of repair in transcribed genes of chicken cells is about one or two lesions removed per 106 base pairs of DNA per minute during the first 3 hr after irradiation.

Original languageEnglish (US)
Pages (from-to)752-756
Number of pages5
JournalVirology
Volume112
Issue number2
DOIs
StatePublished - Jul 30 1981

Bibliographical note

Funding Information:
the American Cancer Society Grant, VC-70. P.B.H. was supported by fellowships from the Damon Runyon-Walter Winchell Cancer Fund and a National Service Research Award from the National Institutes of Health.

Fingerprint Dive into the research topics of 'Repair of lesions which cause premature termination of transcription in chicken embryo cells irradiated with ultraviolet light'. Together they form a unique fingerprint.

Cite this