Repair of lesions which cause premature termination of transcription in chicken embryo cells irradiated with ultraviolet light

Perry B. Hackett; Harold E. Varmus; J. Michael Bishop

doi:10.1016/0042-6822(81)90320-2

Repair of lesions which cause premature termination of transcription in chicken embryo cells irradiated with ultraviolet light

Perry B. Hackett, Harold E. Varmus, J. Michael Bishop

Genetics, Cell Biology and Development (CBS)

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

The dose-response curves for the synthesis of rRNA and avian sarcoma virus mRNA, after irradiation of virus-infected chicken embryo fibrolast cells, have shoulders (P. B. Hackett, H. E. Varmus, and J. M. Bishop, 1981. Virology, 112, 714-728). We tested the hypothesis that the shoulders were the result of dark repair of transcription-terminating lesions after irradiation by measuring the amounts of rRNA synthesized at various times after the uv dose was applied. Our data support the hypothesis and indicated that the rate of repair in transcribed genes of chicken cells is about one or two lesions removed per 10⁶ base pairs of DNA per minute during the first 3 hr after irradiation.

Original language	English (US)
Pages (from-to)	752-756
Number of pages	5
Journal	Virology
Volume	112
Issue number	2
DOIs	https://doi.org/10.1016/0042-6822(81)90320-2
State	Published - Jul 30 1981

Bibliographical note

Funding Information:
the American Cancer Society Grant, VC-70. P.B.H. was supported by fellowships from the Damon Runyon-Walter Winchell Cancer Fund and a National Service Research Award from the National Institutes of Health.

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title = "Repair of lesions which cause premature termination of transcription in chicken embryo cells irradiated with ultraviolet light",

abstract = "The dose-response curves for the synthesis of rRNA and avian sarcoma virus mRNA, after irradiation of virus-infected chicken embryo fibrolast cells, have shoulders (P. B. Hackett, H. E. Varmus, and J. M. Bishop, 1981. Virology, 112, 714-728). We tested the hypothesis that the shoulders were the result of dark repair of transcription-terminating lesions after irradiation by measuring the amounts of rRNA synthesized at various times after the uv dose was applied. Our data support the hypothesis and indicated that the rate of repair in transcribed genes of chicken cells is about one or two lesions removed per 106 base pairs of DNA per minute during the first 3 hr after irradiation.",

author = "Hackett, {Perry B.} and Varmus, {Harold E.} and Bishop, {J. Michael}",

note = "Funding Information: the American Cancer Society Grant, VC-70. P.B.H. was supported by fellowships from the Damon Runyon-Walter Winchell Cancer Fund and a National Service Research Award from the National Institutes of Health.",

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AU - Hackett, Perry B.

AU - Varmus, Harold E.

AU - Bishop, J. Michael

N1 - Funding Information: the American Cancer Society Grant, VC-70. P.B.H. was supported by fellowships from the Damon Runyon-Walter Winchell Cancer Fund and a National Service Research Award from the National Institutes of Health.

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N2 - The dose-response curves for the synthesis of rRNA and avian sarcoma virus mRNA, after irradiation of virus-infected chicken embryo fibrolast cells, have shoulders (P. B. Hackett, H. E. Varmus, and J. M. Bishop, 1981. Virology, 112, 714-728). We tested the hypothesis that the shoulders were the result of dark repair of transcription-terminating lesions after irradiation by measuring the amounts of rRNA synthesized at various times after the uv dose was applied. Our data support the hypothesis and indicated that the rate of repair in transcribed genes of chicken cells is about one or two lesions removed per 106 base pairs of DNA per minute during the first 3 hr after irradiation.

AB - The dose-response curves for the synthesis of rRNA and avian sarcoma virus mRNA, after irradiation of virus-infected chicken embryo fibrolast cells, have shoulders (P. B. Hackett, H. E. Varmus, and J. M. Bishop, 1981. Virology, 112, 714-728). We tested the hypothesis that the shoulders were the result of dark repair of transcription-terminating lesions after irradiation by measuring the amounts of rRNA synthesized at various times after the uv dose was applied. Our data support the hypothesis and indicated that the rate of repair in transcribed genes of chicken cells is about one or two lesions removed per 106 base pairs of DNA per minute during the first 3 hr after irradiation.

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Repair of lesions which cause premature termination of transcription in chicken embryo cells irradiated with ultraviolet light

Abstract

Bibliographical note

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