Repair of DNA loops involves DNA-mismatch and nucleotide-excision repair proteins

D. T. Kirkpatrick, T. D. Petes

Research output: Contribution to journalArticle

132 Scopus citations

Abstract

A number of enzymes recognize and repair DNA lesions. The DNA-mismatch repair system corrects base-base mismatches and small loops, whereas the nucleotide-excision repair system removes pyrimidine dimers and other helix- distorting lesions. DNA molecules with mismatches or loops can arise as a consequence of heteroduplex formation during meiotic recombination. In the yeast Saccharomyces cerevisiae, repair of mismatches results in gene conversion or restoration, and failure to repair the mismatch results in post-meiotic segregation (PMS). The ratio of gene-conversion to PMS events reflects the efficiency of DNA repair. By examining the PMS patterns in yeast strains heterozygous for a mutant allele with a 26-base-pair insertion, we find that the repair of 26-base loops involves Msh2 (a DNA-mismatch repair protein) and Rad1 (a protein required for nucleotide-excision repair).

Original languageEnglish (US)
Pages (from-to)929-931
Number of pages3
JournalNature
Volume387
Issue number6636
DOIs
StatePublished - Jul 23 1997

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