REMI mutagenesis and identification of pathogenic mutants in blast fungus (Magnaporthe grisea).

Shujun Liu, R. Wei, T. Arie, I. Yamaguchi

Research output: Contribution to journalArticle

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Abstract

Blast fungus' mutant, which is very difficult to obtain, is the key substance in the study of the relation between pathogen and plant. Rice-Aichi-ashahi was used as the host and plasmid pBF101 or pCSN43 as foreign DNA fragment, and REMI mutagenesis was applied to tag the pathogenic sequence in rice blast fungus M.grisea. Three mutants (R2H65, R2H69 and R2B1565) related to pathogenicity were recovered from several hundreds of transformants. R2H69 and R2H65 produced merely strange conidia which could not form appressorium or yield abnormals, and pathogenicity test further confirmed that they were unable to cause disease. R2B1565 possesses the same phenotypes as those of the wild type except that the ability of causing disease was much reduced.

Original languageEnglish (US)
Pages (from-to)133-139
Number of pages7
JournalChinese Journal of Biotechnology
Volume14
Issue number3
StatePublished - 1998
Externally publishedYes

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